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In Vitro Effects of DuP 753, a Nonpeptide Angiotensin II Receptor Antagonist, on Human Platelets and Rat Vascular Smooth Muscle Cells

Burnier, Michel ; Centeno, Gabriel ; Grouzmann, Eric ; Walker, Philippe ; Waeber, Bernard ; Brunner, Hans R.

In: American Journal of Hypertension, 1991, vol. 4, no. 5_Pt_1, p. 438-443

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    Titre
    • In Vitro Effects of DuP 753, a Nonpeptide Angiotensin II Receptor Antagonist, on Human Platelets and Rat Vascular Smooth Muscle Cells
    Auteur
    • Burnier, Michel. The Hypertension Division and Cardiovascular Research Group, University Hospital, Lausanne, Switzerland..
    • Centeno, Gabriel. The Hypertension Division and Cardiovascular Research Group, University Hospital, Lausanne, Switzerland..
    • Grouzmann, Eric. The Hypertension Division and Cardiovascular Research Group, University Hospital, Lausanne, Switzerland..
    • Walker, Philippe. The Hypertension Division and Cardiovascular Research Group, University Hospital, Lausanne, Switzerland..
    • Waeber, Bernard. The Hypertension Division and Cardiovascular Research Group, University Hospital, Lausanne, Switzerland..
    • Brunner, Hans R.. The Hypertension Division and Cardiovascular Research Group, University Hospital, Lausanne, Switzerland..
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • American Journal of Hypertension, 1991, vol. 4, no. 5_Pt_1, p. 438-443. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:298619
    Summary
    These experiments were designed to assess the ability of the new nonpeptide angiotensin II antagonist DuP 753 to inhibit the binding and, particularly, to antagonize the cellular response to angiotensin II in human platelets and primary cultures of rat aortic smooth muscle cells (SMC). The binding of 125I-angiotensin II was competitively inhibited by DuP 753 with a 50% binding inhibition (IC50) of 5 to 6 × 10—8 mol/L in platelets and 1 × 10—8 mol/L in vascular SMC as compared to an IC50 of 5 to 7.5 × 10×9 mol/L with nonlabeled angiotensin II. In vascular SMC, DuP 753 completely abolished the effects of angiotensin II on 45CaCl2 efflux and 45CaCl2 uptake. Moreover, in these latter cells, DuP 753 prevented the angiotensin II but not the vasopressin induced increase in cytosolic calcium. These results demonstrate that DuP 753 competes with angiotensin II binding to its receptor in both animal and human cells and selectively blocks the cellular response to angiotensin II. Am J Hypertens 1991;4:438-443