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SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements

Nayler, Oliver ; Strätling, Wolf ; Bourquin, Jean-Pierre ; Stagljar, Igor ; Lindemann, Lothar ; Jasper, Heinrich ; Hartmann, Annette M. ; Fackelmayer, Frank O. ; Ullrich, Axel ; Stamm, Stefan

In: Nucleic Acids Research, 1998, vol. 26, no. 15, p. 3542-3549

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    Title
    • SAF-B protein couples transcription and pre-mRNA splicing to SAR/MAR elements
    Author
    • Nayler, Oliver. Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    • Strätling, Wolf. Institute for Physiological Chemistry, Martinistrasse 52, 20246 Hamburg, Germany
    • Bourquin, Jean-Pierre. Institute for Molecular Biology, Department II, University of Zürich, CH-8057 Zürich, Switzerland
    • Stagljar, Igor. Institute for Molecular Biology, Department II, University of Zürich, CH-8057 Zürich, Switzerland
    • Lindemann, Lothar. Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    • Jasper, Heinrich. Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    • Hartmann, Annette M.. Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    • Fackelmayer, Frank O.. Department of Biology, University of Konstanz, D-78457 Konstanz, Germany
    • Ullrich, Axel. Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    • Stamm, Stefan. Max-Planck Institute of Neurobiology, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Nucleic Acids Research, 1998, vol. 26, no. 15, p. 3542-3549. Oxford University Press
    Other Version
    Classification
    • Health
    OAI-PMH Identifier
    • oai:doc.rero.ch:298399
    Summary
    Interphase chromatin is arranged into topologically separated domains comprising gene expression and replication units through genomic sequence elements, so-called MAR or SAR regions (for matrix- or scaffoldassociating regions). S/MAR regions are located near the boundaries of actively transcribed genes and were shown to influence their activity. We show that scaffold attachment factor B (SAF-B), which specifically binds to S/MAR regions, interacts with RNA polymerase II (RNA pol II) and a subset of serine-/arginine-rich RNA processing factors (SR proteins). SAF-B localized to the nucleus in a speckled pattern that coincided with the distribution of the SR protein SC35. Furthermore, we show that overexpressed SAF-B induced an increase of the 10S splice product using an E1A reporter gene and repressed the activity of an S/MAR flanked CAT reporter gene construct in vivo. This indicates an association of SAF-B with SR proteins and components of the transcription machinery. Our results describe the coupling of a chromatin organizing S/MAR element with transcription and pre-mRNA processing components and we propose that SAF-B serves as a molecular base to assemble a ‘transcriptosome complex' in the vicinity of actively transcribed genes