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Characterization of the non-functional Fas ligand of gld mice

Hahne, Michael ; Peitsch, Manuel C. ; Irmler, Martin ; Schröter, Michael ; Lowin, Bente ; Rousseau, Marga ; Bron, Claude ; Renno, Toufic ; French, Lars ; Tschopp, Jürg

In: International Immunology, 1995, vol. 7, no. 9, p. 1381-1386

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    Titel
    • Characterization of the non-functional Fas ligand of gld mice
    Autor
    • Hahne, Michael. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    • Peitsch, Manuel C.. Glaxo Institute for Molecular Biology Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland
    • Irmler, Martin. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    • Schröter, Michael. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    • Lowin, Bente. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    • Rousseau, Marga. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    • Bron, Claude. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    • Renno, Toufic. Ludwig Institute for Cancer Research, BIL Research Center Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland
    • French, Lars. Division of Dermatology, Geneva University Hospital CH-1211 Geneva 14, Switzerland
    • Tschopp, Jürg. Institute of Biochemistry, University of Lausanne and, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland, Chemin des Aulx 14, CH-1228 Plan-les-Ouates, Switzerland, CH-1211 Geneva 14, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • International Immunology, 1995, vol. 7, no. 9, p. 1381-1386. Oxford University Press
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:297277
    Summary
    Mice homozygous for either the gld or Ipr mutation develop autoimmune diseases and progressive lymphadenopathy. The Ipr mutation Is characterized by the absence of unctional Fas, whereas gld mice exhibit an inactive FasL due to a point mutation proximal to the extracellular C-terminus. The structural repercussions of this amino acid substitution remain unknown. Here we report that FasL Is expressed at similar levels on the surface of activated T lymphocytes from gld and wild-type mice. Using a polyclonal anti-FasL antibody, Indistinguishable amounts of a 40 kDa protein are detected In both gld and wild-type splenocytes. The molecular model of FasL, based on the known structure of TNF-α, predicts that the Phe→Leu gld mutation is located at the protomer interface which Is close to the FasR Interaction site. We conclude that the gld mutation allows normal FasL biosynthesis, surface expression and ollgomerlzatlon, but induces structural alterations to the Fas binding region leading to the phenotypic changes observed