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Metagenomic analysis of the impact of nitrofurantoin treatment on the human faecal microbiota

Vervoort, Jascha ; Xavier, Basil Britto ; Stewardson, Andrew ; Coenen, Samuel ; Godycki-Cwirko, Maciek ; Adriaenssens, Niels ; Kowalczyk, Anna ; Lammens, Christine ; Harbarth, Stephan ; Goossens, Herman ; Malhotra-Kumar, Surbhi

In: Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 7, p. 1989-1992

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    Titre
    • Metagenomic analysis of the impact of nitrofurantoin treatment on the human faecal microbiota
    Auteur
    • Vervoort, Jascha. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    • Xavier, Basil Britto. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    • Stewardson, Andrew. Infection Control Program, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland
    • Coenen, Samuel. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    • Godycki-Cwirko, Maciek. Centre for Family and Community Medicine, Medical University of Lodz, Lodz, Poland
    • Adriaenssens, Niels. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    • Kowalczyk, Anna. Centre for Family and Community Medicine, Medical University of Lodz, Lodz, Poland
    • Lammens, Christine. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    • Harbarth, Stephan. Infection Control Program, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland
    • Goossens, Herman. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    • Malhotra-Kumar, Surbhi. Department of Medical Microbiology, Universiteit Antwerpen, Antwerp, Belgium
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 7, p. 1989-1992. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:297014
    Summary
    Objectives The objective was to study changes in the faecal microbiota of patients with uncomplicated urinary tract infections (UTIs) treated with nitrofurantoin and of non-treated healthy controls using 16S rRNA analysis. Methods Serial stool samples were collected from patients receiving nitrofurantoin treatment at different timepoints [before treatment (day 1; T1), within 48 h of end of treatment (days 5-15; T2) and 28 days after treatment (days 31-43; T3)], as well as from healthy controls. Direct DNA extraction (PowerSoil DNA Isolation Kit, MoBio Laboratories, Carlsbad, CA, USA) from stool samples was followed by pyrosequencing (454 GS FLX Titanium) of the V3-V5 region of the bacterial 16S rRNA gene. Results Among UTI patients, mean proportions of the Actinobacteria phylum increased by 19.6% in the first follow-up sample (T2) in comparison with the pretreatment baseline stool sample (T1) (P = 0.026). However, proportions of Actinobacteria reversed to ‘normal' pre-antibiotic levels, with a mean difference of 1.0% compared with baseline proportions, in the second follow-up sample (T3). The increase in Actinobacteria was specifically due to an increase in the Bifidobacteriaceae family (Bifidobacterium genus), which constituted 81.0% (95% CI ±7.4%) of this phylum. Conclusions No significant impact was observed other than a temporary increase in the beneficial Bifidobacterium genus following nitrofurantoin treatment, which supports its reintroduction into clinical use