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Role of Bacterial Exopolymers and Host Factors on Adherence and Phagocytosis of Staphylococcus aureus in Foreign Body Infection

Falcieri, Elisabetta ; Vaudaux, Pierre ; Huggler, Elzbieta ; Lew, Daniel ; Waldvogel, Francis

In: Journal of Infectious Diseases, 1987, vol. 155, no. 3, p. 524-531

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    Titel
    • Role of Bacterial Exopolymers and Host Factors on Adherence and Phagocytosis of Staphylococcus aureus in Foreign Body Infection
    Autor
    • Falcieri, Elisabetta. Department of Morphology, University Medical Center, and the Division of infectious Diseases, Department of Medicine, University Hospital, Geneva, Switzerland
    • Vaudaux, Pierre. Department of Morphology, University Medical Center, and the Division of infectious Diseases, Department of Medicine, University Hospital, Geneva, Switzerland
    • Huggler, Elzbieta. Department of Morphology, University Medical Center, and the Division of infectious Diseases, Department of Medicine, University Hospital, Geneva, Switzerland
    • Lew, Daniel. Department of Morphology, University Medical Center, and the Division of infectious Diseases, Department of Medicine, University Hospital, Geneva, Switzerland
    • Waldvogel, Francis. Department of Morphology, University Medical Center, and the Division of infectious Diseases, Department of Medicine, University Hospital, Geneva, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Journal of Infectious Diseases, 1987, vol. 155, no. 3, p. 524-531. The University of Chicago Press
    Andere elektronische Ausgabe
    Klassifikation
    • Gesundheit
    OAI-PMH-ID
    • oai:doc.rero.ch:295949
    Summary
    Using a previously developed guinea pig model of foreign body infection, we examined ultrastructural and functional surface alterations of Staphylococcus aureus strain Wood 46 during the early phase of infection. Exopolymer-free bacteria were prepared and inoculated into subcutaneously implanted tissue cages. After three hours, the bacteria showed abundant capsular and intercellular exopolymers, which were visualized by transmission electron microscopy. Exopolymers were also produced by S. aureus exposed in vitro to fluid from the tissue cage. In contrast, human serum albumin prevented exopolymer production by S. aureus. The influence of exopolymers on the susceptibility of S. aureus to ingestion and phagocytic killing by neutrophils was tested in vitro and found to be negligible. Furthermore, adherence of S. aureus to fibronectin-coated surfaces was unaffected by the presence or absence of exopolymers. Thus, in our experimental model, exopolymers are produced early during the onset of infection, but they have little impact on adherence and phagocytosis