Fulltext

Cell-Associated HIV-1 RNA in Blood as Indicator of Virus Load in Lymph Nodes

Yerly, S. ; Rutschmann, O. T. ; Opravil, M. ; Marchal, F. ; Hirschel, B. ; Perrin, L.

In: The Journal of Infectious Diseases, 1999, vol. 180, no. 3, p. 850-853

Aggiungi alla tua lista
    Titre
    • Cell-Associated HIV-1 RNA in Blood as Indicator of Virus Load in Lymph Nodes
    Auteur
    • Yerly, S.. Laboratory of Virology
    • Rutschmann, O. T.. AIDS Center, Division of Infectious Diseases
    • Opravil, M.. Division of Infectious Diseases, Zurich University Hospital, Zurich, Switzerland
    • Marchal, F.. Clinic ENT, Geneva University Hospital, Geneva
    • Hirschel, B.. AIDS Center, Division of Infectious Diseases
    • Perrin, L.. Laboratory of Virology
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • The Journal of Infectious Diseases, 1999, vol. 180, no. 3, p. 850-853. The University of Chicago Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:295287
    Summary
    We have developed sensitive assays for viremia and cell-associated human immunodeficiency virus type 1 (HIV-1) RNA and DNA to assess the predictive value of virological parameters determined in blood for virus load in lymph nodes (LNs). Eighteen patients were included; 13 received stavudine/didanosine/hydroxyurea and 5 stavudine/didanosine, and all had viremia <500 copies/mL for >3 months. At the time of LN biopsy (median, 10 months), the median viremia was 2.09 log copies/mL (range, <0.70-3.34). Cell-associated HIV-1 RNA and DNA were detectable in blood and LNs of all patients. The median cell-associated RNA and DNA were 2.16 log copies/106 cells and 2.60 log copies/106 cells in blood versus 4.31 log RNA copies/106 cells and 3.26 log DNA copies/106 cells in LNs. Regression analysis shows that, in treated patients with sustained low viremia, cell-associated RNA and DNA in blood are better predictors of virus load in LNs than viremia