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Dissection of gene regulatory networks in embryonic stem cells by means of high-throughput sequencing

Beisel, Christian ; Paro, Renato

In: Biological Chemistry, 2009, vol. 390, no. 11, p. 1139-1144

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    Title
    • Dissection of gene regulatory networks in embryonic stem cells by means of high-throughput sequencing
    Author
    • Beisel, Christian. Department Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland
    • Paro, Renato. Department Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Biological Chemistry, 2009, vol. 390, no. 11, p. 1139-1144. Walter de Gruyter
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:294816
    Summary
    Transcription factor regulation of gene expression and chromatin-controlled epigenetic memory systems are closely cooperating in establishing the pluripotent state of embryonic stem (ES) cells and maintaining cell fate decisions throughout development of an organism. A thorough understanding of the regulatory transcriptional circuitry that rules the underlying plastic yet heritable gene expression programs in ES cells is of great importance. With the advent of next-generation sequencing technologies facilitating the quantitative assessment of functional genomics assays it is now feasible to interrogate transcription networks at a genome-wide scale. Here, we discuss the application of next-generation sequencing in elucidating the molecular mechanisms underlying ES cell function