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Effect of ampicillin-sulbactam on clinical capillary zone electrophoresis of serum proteins

Siede, Dominik ; Möller, Helgard ; Siede, Werner H. ; Regeniter, Axel

In: Clinical Chemistry and Laboratory Medicine, 2008, vol. 46, no. 10, p. 1468-1469

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    Titre
    • Effect of ampicillin-sulbactam on clinical capillary zone electrophoresis of serum proteins
    Auteur
    • Siede, Dominik. Institut für Laboratoriums- und Transfusionsmedizin, Klinikum-Lippe GmbH, Detmold, Germany
    • Möller, Helgard. Institut für Laboratoriums- und Transfusionsmedizin, Klinikum-Lippe GmbH, Detmold, Germany
    • Siede, Werner H.. Institut für Laboratoriums- und Transfusionsmedizin, Klinikum-Lippe GmbH, Detmold, Germany
    • Regeniter, Axel. Labormedizin, Universitätsspital Basel, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Clinical Chemistry and Laboratory Medicine, 2008, vol. 46, no. 10, p. 1468-1469. Walter de Gruyter
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:294625
    Summary
    Background: Capillary zone electrophoresis (CZE) is a well-accepted automated method used to separate serum proteins and detect monoclonal components. CZE uses ultraviolet detection at 214nm to directly quantify proteins via peptide bonds. Any substance that absorbs at 214nm and is present in serum can potentially interfere with CZE analysis. This has been reported for radio-contrast media and antibiotics. Methods: Here we describe a peak on the anode side of the α2-globulin fraction caused by the antibiotic ampicillin-sulbactam (Unacid®). Results and conclusions: Extra peaks that can be misinterpreted as monoclonal components can be present in almost all electrophoretic fractions of CZE. Immunosubtraction or immunofixation is always required to rule out these conditions. Clin Chem Lab Med 2008;46:1468-9