Fulltext

Long term accuracy of fluorescence polarization immunoassays for gentamicin, tobramycin, netilmicin and vancomycin

Joos, Beda ; Lüthy, Ruedi ; Blaser, Jürg

In: Journal of Antimicrobial Chemotherapy, 1989, vol. 24, no. 5, p. 797-803

Zum persönliche Liste hinzufügen
    Titel
    • Long term accuracy of fluorescence polarization immunoassays for gentamicin, tobramycin, netilmicin and vancomycin
    Autor
    • Joos, Beda. Division of Infectious Diseases. Department of Medicine, University Hospital, CH-8091 Zurich, Switzerland
    • Lüthy, Ruedi. Division of Infectious Diseases. Department of Medicine, University Hospital, CH-8091 Zurich, Switzerland
    • Blaser, Jürg. Division of Infectious Diseases. Department of Medicine, University Hospital, CH-8091 Zurich, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Journal of Antimicrobial Chemotherapy, 1989, vol. 24, no. 5, p. 797-803. Oxford University Press
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:293350
    Summary
    External quality control was performed during six years to determine the accuracy over time of the Abbott TDx fluorescence polarization system for assaying antibiotics. Unknown spiked serum samples of gentamicin, tobramycin, netilmicin and vancomycin were provided monthly by the British national external quality assessment scheme. Comparison of the 209 assay results with the target concentrations showed good correlations in all four assays. No significant deviations from linearity, from slope 1·0, and from intercept 0·0 were detected by regression analysis. Relative deviations were <10% and <15% for 78% and 90% of all specimens, respectively. On an average the same calibration curves could be used over a period of 19 weeks . Fluorescence polarization immunoassays provided rapid and reliable results over the entire study period