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Detecting malaria sporozoites in live, field-collected mosquitoes

Habluetzel, A. ; Merzagora, L. ; Jenni, L. ; Betschart, B. ; Rotigliano, G. ; Esposito, F.

In: Transactions of The Royal Society of Tropical Medicine and Hygiene, 1992, vol. 86, no. 2, p. 138-140

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    Titel
    • Detecting malaria sporozoites in live, field-collected mosquitoes
    Autor
    • Habluetzel, A.. Department of Molecular, Cell, and Animal Biology, University of Camerino, Via Camerini 2, 62032 Camerino (MC, Italy
    • Merzagora, L.. Institute of Parasitology, ‘La Sapienza' University, Rome, Italy
    • Jenni, L.. Swiss Tropical Institute, CH-4002 Basel, Switzerland
    • Betschart, B.. Swiss Tropical Institute, CH-4002 Basel, Switzerland
    • Rotigliano, G.. Italian Ministry for Foreign Affairs, Directorate General for Co-operation in Development, and Institute of Tropical Diseases (3rd chair), ‘La Sapienza' University, Rome, Italy
    • Esposito, F.. Department of Molecular, Cell, and Animal Biology, University of Camerino, Via Camerini 2, 62032 Camerino (MC, Italy
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Transactions of The Royal Society of Tropical Medicine and Hygiene, 1992, vol. 86, no. 2, p. 138-140. Royal Society of Tropical Medicine and Hygiene
    Andere elektronische Ausgabe
    Klassifikation
    • Gesundheit
    OAI-PMH-ID
    • oai:doc.rero.ch:291978
    Summary
    A method is described for identifying malaria-infected mosquitoes, without killing them or hampering their fitness. Individual mosquitoes were induced to salivate on coverslips, and sporozoites, deposited on the glass surface, were visualized by Giemsa staining. Of 21 mosquitoes found to contain sporozoites by salivary gland dissection, 13 had delivered sporozoites on coverslips. A positive correlation was found between the amount of saliva expelled and ejection of sporozoites, indicating that the sensitivity of the method may be increased by improving the probing behaviour of the mosquitoes. The procedure described may be suitable for selecting infected mosquitoes which are able to eject sporozoites during probing. Being applicable to wild Anopheles and to large numbers of mosquitoes, the method lends itself for use in field studies on malaria