Fulltext

Physiological Aspects of Cluster Root Function and Development in Phosphorus-deficient White Lupin (Lupinus albus L.)

Neumann, Günter ; Massonneau, Agnès ; Langlade, Nicolas ; Dinkelaker, Barbara ; Hengeler, Christine ; Römheld, Volker ; Martinoia, Enrico

In: Annals of Botany, 2000, vol. 85, no. 6, p. 909-919

Ajouter à la liste personnelle
    Titre
    • Physiological Aspects of Cluster Root Function and Development in Phosphorus-deficient White Lupin (Lupinus albus L.)
    Auteur
    • Neumann, Günter. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    • Massonneau, Agnès. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    • Langlade, Nicolas. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    • Dinkelaker, Barbara. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    • Hengeler, Christine. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    • Römheld, Volker. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    • Martinoia, Enrico. Institut für Planzenernährung (330), Universität Hohenheim, D-70593, Stuttgart, Germany Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Annals of Botany, 2000, vol. 85, no. 6, p. 909-919. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:291053
    Summary
    Cluster root formation in white lupin (Lupinus albus L.) is induced mainly by phosphorus (P) starvation, and seems to be regulated by the endogenous P status of the plant. Increased formation of cluster roots, when indole acetic acid is supplied to the growth medium of P sufficient plants, and inhibitory effects of kinetin application suggest the involvement of endogenous phytohormones (auxins and cytokinins), which may act in an antagonistic manner in the P-starvation response. Phosphorus deficiency-induced adaptations of white lupin, involved in P acquisition and mobilization of sparingly available P sources, are predominantly confined to the cluster roots, and moreover to distinct stages during their development. Increased accumulation and exudation of citrate and a concomitant release of protons were found to be mainly restricted to mature root clusters after prolonged culture (3-4 weeks) under P-deficient conditions. Inhibition of citrate exudation by exogenous application of anion channel antagonists such as ethacrynic- and anthracene-9-carboxylic acids may indicate involvement of an anion channel. Phosphorus deficiency-induced accumulation and subsequent exudation of citric acid seems to be a consequence of both enhanced biosynthesis and reduced turnover of citric acid in the cluster root tissue, indicated by enhanced expression of sucrose synthase, fructokinase, phosphoglucomutase, phosphoenol-pyruvate carboxylase, but reduced activity of aconitase and slower root respiration. The release of acid phosphatase and of phenolic compounds (isoflavonoids) as well as the induction of a putative high-affinity P uptake system was more highly expressed in juvenile, mature and even senescent cluster regions than in apical zones of non-proteoid roots. An AFLP-cDNA library for cluster root-specific gene expression was constructed to assist in the identification of further genes involved in cluster root development. Copyright 2000 Annals of Botany Company