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Regional IFNγ expression is insufficient for efficacious control of food-borne bacterial pathogens at the gut epithelial barrier

Gajendran, Nadesan ; Mittrücker, Hans-Willi ; Bordasch, Karin ; Heinemann, Ellen ; Koch, Markus ; Kaufmann, Stefan H. E.

In: International Immunology, 2007, vol. 19, no. 9, p. 1075-1081

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    Titre
    • Regional IFNγ expression is insufficient for efficacious control of food-borne bacterial pathogens at the gut epithelial barrier
    Auteur
    • Gajendran, Nadesan. Present address: Institute of Physiology, Department of Biomedicine Pharmazentrum/Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland
    • Mittrücker, Hans-Willi. Present address: Institute for Immunology, University Medical Center Hamburg-Eppendorf, Martinistraße 52, D-20246 Hamburg, Germany
    • Bordasch, Karin. Max Planck Institute for Infection Biology, Department of Immunology, Charitéplatz 1, Berlin, 10117 Germany
    • Heinemann, Ellen. Max Planck Institute for Infection Biology, Department of Immunology, Charitéplatz 1, Berlin, 10117 Germany
    • Koch, Markus. Max Planck Institute for Infection Biology, Department of Immunology, Charitéplatz 1, Berlin, 10117 Germany
    • Kaufmann, Stefan H. E.. Max Planck Institute for Infection Biology, Department of Immunology, Charitéplatz 1, Berlin, 10117 Germany
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • International Immunology, 2007, vol. 19, no. 9, p. 1075-1081. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:291022
    Summary
    IFNγ is critical for host defence against various food-borne pathogens including Salmonella enterica and Listeria monocytogenes, the causative agents of salmonellosis and listeriosis, respectively. We investigated the impact of regional IFNγ expression at the intestinal epithelial barrier on host invasion by salmonellae and listeriae following oral challenge. Transgenic mice (IFNγ-gut), generated on an IFNγ knock-out (KO) background, selectively expressed IFNγ in the gut driven by the modified liver fatty acid-binding protein (Fabpl4× at −132) promoter. Infections with attenuated S. enterica Typhimurium or with L. monocytogenes did not differ significantly in IFNγ-KO, IFNγ-gut and wild-type mice. Further, Listeria-specific CD4+ and CD8+ T cells were not altered in IFNγ-gut mice. Thus, this model indicates that local IFNγ expression by non-immunological cells in the distal part of the small intestine, caecum and colon is insufficient for prevention of gut penetration by S. enterica Typhimurium and L. monocytogenes