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A rhoptry antigen of Plasmodium falciparum is protective in Saimiri monkeys

Ridley, R. G. ; Takacs, B. ; Etlinger, H. ; Scaife, J. G.

In: Parasitology, 1990, vol. 101, no. 2, p. 187-192

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    Titel
    • A rhoptry antigen of Plasmodium falciparum is protective in Saimiri monkeys
    Autor
    • Ridley, R. G.. Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Switzerland
    • Takacs, B.. Central Research Units, F Hoffman-La Roche Limited, CH-4002 Basel, Switzerland
    • Etlinger, H.. Central Research Units, F Hoffman-La Roche Limited, CH-4002 Basel, Switzerland
    • Scaife, J. G.. Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Parasitology, 1990, vol. 101, no. 2, p. 187-192. Cambridge University Press
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:290771
    Summary
    A non-polymorphic antigen associated with the rhoptry organelles of Plasmodium falciparum has been purified by immuno-affinity chromatography. The antigen, RAP-1 (rhoptry associated protein-1). which is defined by monoclonal antibodies which inhibit parasite growth in vitro, is a multi-component antigen consisting of four major proteins of 80, 65, 42 and 40 kDa and two minor proteins of 77 and 70 kDa. These proteins were electro-eluted from preparative sodium dodecyl sulphate polyacrylamide gels and protected Saimiri sciureus monkeys from a lethal blood-stage infection of P. falciparum malaria. Sera from the protected animals recognized only proteins of the RAP-1 antigen when used to probe a Western blot of total parasite protein extract, confirming that RAP-1 is responsible for eliciting the protective immune response