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Nitrite Generation in Interleukin-4—Treated Human Macrophage Cultures Does Not Involve the Nitric Oxide Synthase Pathway

Schneemann, M. ; Schoedon, G. ; Linscheid, P. ; Walter, R. ; Blau, N. ; Schaffner, A.

In: Journal of Infectious Diseases, 1997, vol. 175, no. 1, p. 130-135

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    Title
    • Nitrite Generation in Interleukin-4—Treated Human Macrophage Cultures Does Not Involve the Nitric Oxide Synthase Pathway
    Author
    • Schneemann, M.. Clinical Mycology Laboratory, Division ofInfectious Diseases, Department of Medicine, University of Zurich Medical School, and Division of Clinical Chemistry, Department of Pediatrics, University of Zurich Children's Hospital, Zurich Switzerland
    • Schoedon, G.. Clinical Mycology Laboratory, Division ofInfectious Diseases, Department of Medicine, University of Zurich Medical School, and Division of Clinical Chemistry, Department of Pediatrics, University of Zurich Children's Hospital, Zurich Switzerland
    • Linscheid, P.. Clinical Mycology Laboratory, Division ofInfectious Diseases, Department of Medicine, University of Zurich Medical School, and Division of Clinical Chemistry, Department of Pediatrics, University of Zurich Children's Hospital, Zurich Switzerland
    • Walter, R.. Clinical Mycology Laboratory, Division ofInfectious Diseases, Department of Medicine, University of Zurich Medical School, and Division of Clinical Chemistry, Department of Pediatrics, University of Zurich Children's Hospital, Zurich Switzerland
    • Blau, N.. Clinical Mycology Laboratory, Division ofInfectious Diseases, Department of Medicine, University of Zurich Medical School, and Division of Clinical Chemistry, Department of Pediatrics, University of Zurich Children's Hospital, Zurich Switzerland
    • Schaffner, A.. Clinical Mycology Laboratory, Division ofInfectious Diseases, Department of Medicine, University of Zurich Medical School, and Division of Clinical Chemistry, Department of Pediatrics, University of Zurich Children's Hospital, Zurich Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Journal of Infectious Diseases, 1997, vol. 175, no. 1, p. 130-135. The University of Chicago Press
    Other Version
    Classification
    • Health
    OAI-PMH Identifier
    • oai:doc.rero.ch:290158
    Summary
    The search continues for high-output nitric oxide biosynthesis in human macrophages analogous to murine phagocytes. Recently, generation of nitrite in culture supernatants of human macrophages exposed to interferon-γ and interleukin-4 (IFN-γ/IL-4) was reported. The present study reproduces these findings and shows that L-arginine is not consumed and L-citrulline is not produced during this process. Furthermore, the biosynthesis of the obligatory cofactor tetrahydrobiopterin is not coinduced. These biochemical data provide support against a nitric oxide synthase contribution to nitrite accumulation. Nitrite was generated from nitrate salts even in cell-free media. Nitric oxide synthase activity but not nitrate reduction depended on molecular oxygen. Nitrite accumulation in experiments with IFN-γ/IL-4 in human monocytes appears to be an in vitro artifact produced by nitrate-reducing activities contained in cytokine preparations