Fulltext

Examination of Gould's modified S1 (mS1) selective medium and Angle's non-selective medium for describing the diversity of Pseudomonas spp. in soil and root environments

Tarnawski, Sonia ; Hamelin, Jérôme ; Locatelli, Laurent ; Aragno, Michel ; Fromin, Nathalie

In: FEMS Microbiology Ecology, 2003, vol. 45, no. 2, p. 97-104

Ajouter à la liste personnelle
    Titre
    • Examination of Gould's modified S1 (mS1) selective medium and Angle's non-selective medium for describing the diversity of Pseudomonas spp. in soil and root environments
    Auteur
    • Tarnawski, Sonia. Laboratoire de Microbiologie, Université de Neuchâtel, Case Postale 2, 2007 Neuchâtel, Switzerland
    • Hamelin, Jérôme. Laboratoire de Microbiologie, Université de Neuchâtel, Case Postale 2, 2007 Neuchâtel, Switzerland
    • Locatelli, Laurent. Laboratoire de Microbiologie, Université de Neuchâtel, Case Postale 2, 2007 Neuchâtel, Switzerland
    • Aragno, Michel. Laboratoire de Microbiologie, Université de Neuchâtel, Case Postale 2, 2007 Neuchâtel, Switzerland
    • Fromin, Nathalie. Laboratoire de Microbiologie, Université de Neuchâtel, Case Postale 2, 2007 Neuchâtel, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:289216
    Summary
    Studies on the diversity of environmental culturable Pseudomonas populations are dependent on the isolation procedure. This procedure includes the use of selective media which may influence the recovery of strains and thus the diversity described. In this study, we assessed the use of two agar isolation media for describing the diversity of soil- and root-inhabiting Pseudomonas associated with the perennial grass Molinia coerulea. A total of 382 Pseudomonas strains were recovered on either non-selective Angle's medium, or on Gould's modified S1 (mS1) Pseudomonas-selective medium. Their diversity was assessed by restriction analysis of PCR (polymerase chain reaction)-amplified 16S-23S rDNA internal transcript spacer sequences. The comparison of mS1- and Angle-recovered populations showed that the use of mS1 selective medium led to an underestimation of both Pseudomonas counts and diversity, especially in the soil environment