Université de Fribourg

Antimicrobial Resistance in Escherichia coli

Poirel, Laurent ; Madec, Jean-Yves ; Lupo, Agnese ; Schink, Anne-Kathrin ; Kieffer, Nicolas ; Nordmann, Patrice ; Schwarz, Stefan

In: Microbiology Spectrum, 2018, vol. 6, no. 4, p. -

Multidrug resistance in Escherichia coli has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. E. coli is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic...

Université de Fribourg

Occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in the European survey of carbapenemase-producing Enterobacteriaceae (EuSCAPE): a prospective, multinational study

Grundmann, Hajo ; Glasner, Corinna ; Albiger, Barbara ; Aanensen, David M. ; Tomlinson, Chris T. ; Andrasević, Arjana Tambić ; Cantón, Rafael ; Carmeli, Yehuda ; Friedrich, Alexander W. ; Giske, Christian G. ; Glupczynski, Youri ; Gniadkowski, Marek ; Livermore, David M. ; Nordmann, Patrice ; Poirel, Laurent ; Rossolini, Gian M. ; Seifert, Harald ; Vatopoulos, Alkiviadis ; Walsh, Timothy ; Woodford, Neil ; Monnet, Dominique L. ; the European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group

In: The Lancet Infectious Diseases, 2017, vol. 17, no. 2, p. 153–163

Gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in Europe make it difficult to contain carbapenemase-producing Enterobacteriaceae. We report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase- producing Klebsiella pneumoniae and Escherichia coli in European...

Université de Fribourg

National survey of colistin resistance among carbapenemase-producing Enterobacteriaceae and outbreak caused by colistin-resistant OXA-48-producing Klebsiella pneumoniae , France, 2014

Jayol, Aurélie ; Poirel, Laurent ; Dortet, Laurent ; Nordmann, Patrice

In: Eurosurveillance, 2016, vol. 21, no. 37, p. -

From January 2014 to December 2014, 972 consecutive non-replicate carbapenemase-producing Enterobacteriaceae isolates from colonised or infected patients were collected at the Associated French National Reference Centre as part of the French national survey on antimicrobial resistance. It included 577 Klebsiella spp. (59%), 236 Escherichia coli (24%), 108 Enterobacter spp. (11%), 50...

Université de Fribourg

National survey of colistin resistance among carbapenemase-producing Enterobacteriaceae and outbreak caused by colistin-resistant OXA-48-producing Klebsiella pneumoniae , France, 2014

Jayol, Aurélie ; Poirel, Laurent ; Dortet, Laurent ; Nordmann, Patrice

In: Eurosurveillance, 2016, vol. 21, no. 37, p. -

From January 2014 to December 2014, 972 consecutive non-replicate carbapenemase-producing Enterobacteriaceae isolates from colonised or infected patients were collected at the Associated French National Reference Centre as part of the French national survey on antimicrobial resistance. It included 577 Klebsiella spp. (59%), 236 Escherichia coli (24%), 108 Enterobacter spp. (11%), 50...

Université de Fribourg

Structure of the catalytic domain of the colistin resistance enzyme MCR-1

Stojanoski, Vlatko ; Sankaran, Banumathi ; Prasad, B. V. Venkataram ; Poirel, Laurent ; Nordmann, Patrice ; Palzkill, Timothy

In: BMC Biology, 2016, vol. 14, p. 81

Due to the paucity of novel antibiotics, colistin has become a last resort antibiotic for treating multidrug resistant bacteria. Colistin acts by binding the lipid A component of lipopolysaccharides and subsequently disrupting the bacterial membrane. The recently identified plasmid-encoded MCR-1 enzyme is the first transmissible colistin resistance determinant and is a cause for concern for...

Université de Fribourg

Evaluation of the RAPIDEC® CARBA NP, the Rapid CARB Screen® and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent ; Agathine, Aurélie ; Naas, Thierry ; Cuzon, Gaëlle ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2015, p. dkv213

The objective of this study was the evaluation of the performance of two commercially available biochemical tests for the rapid detection of carbapenemase-producing Enterobacteriaceae compared with a home-made technique.Methods: A collection of 150 enterobacterial isolates, including 132 isolates with decreased susceptibility to at least one carbapenem molecule, were tested for carbapenemase...

Université de Fribourg

Carbapenemase-producing enterobacteriaceae in Europe: a survey among national experts from 39 countries, february 2013

Glasner, C. ; Albiger, B. ; Buist, G. ; Tambić Andrašević, A. ; Canton, R. ; Carmeli, Y. ; Friedrich, A. W. ; Giske, C. G. ; Glupczynski, Y. ; Gniadkowski, M. ; Livermore, D. M. ; Nordmann, Patrice ; Poirel, Laurent ; Rossolini, G. M. ; Seifert, H. ; Vatopoulos, A. ; Walsh, T. ; Woodford, N. ; Donker, T. ; Monnet, D. L. ; Grundmann, H. ; group, the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) working

In: Euro Surveillance, 2013, vol. 18, no. 28, p. 20525

The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a threat to healthcare delivery, although its extent differs substantially from country to country. In February 2013, national experts from 39 European countries were invited to self-assess the current epidemiological situation of CPE in their country. Information about national management of CPE was also reported. The results...

Université de Fribourg

Strategy for a rapid detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent ; Bréchard, Ludivine ; Cuzon, Gaëlle ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 4, p. 2441-2445

A prospective survey was conducted on 862 Enterobacteriaceae with reduced susceptibility to carbapenems. The Carba NP test, UV spectrophotometry and DNA microarray were used for detecting carbapenemase producers, and results compared to PCR and sequencing. The 172 carbapenemase producers were detected using the Carba NP test and UV spectrophotometry whereas DNA microarray failed to detect IMI...