Université de Fribourg

Absence of calretinin protein expression in malignant mesotheliomas from asbestos-exposed NF2+/− mice and mouse mesothelioma cell lines from various mouse strains

Blum, Walter ; Henzi, Thomas ; Châtel-Soulet, Hugues-Etienne ; Pecze, Laszlo ; Wörthmüller Rodriguez, Janine ; Vrugt, Bart ; Schwaller, Beat

In: Biomarker Research, 2018, vol. 6, no. 1, p. 19

Calretinin is the most widespread positive marker for the immunohistochemical identification of malignant mesothelioma (MM) and was proposed to serve as a blood- based biomarker. Functionally, evidence has accumulated that calretinin might be implicated in MM tumorigenesis. We aimed to identify calretinin (CR; Calb2) in murine MM and reactive mesothelial cells in granuloma from...

Université de Fribourg

Biological noise and positional effects influence cell stemness

Blum, Walter ; Henzi, Thomas ; Schwaller, Beat ; Pecze, László

In: Journal of Biological Chemistry, 2018, vol. 293, no. 14, p. 5247–5258

Biological (or cellular) noise is the random quantitative variability of proteins and other molecules in individual, genetically identical cells. As the result of biological noise in the levels of some transcription factors that determine a cell's differentiation status, differentiated cells may dedifferentiate to a stem cell state given a sufficiently long time period. Here, to provide...

Université de Fribourg

Targeting breast cancer cells by MRS1477, a positive allosteric modulator of TRPV1 channels

Nazıroğlu, Mustafa ; Çiğ, Bilal ; Blum, Walter ; Vizler, Csaba ; Buhala, Andrea ; Marton, Annamária ; Katona, Róbert ; Jósvay, Katalin ; Schwaller, Beat ; Oláh, Zoltán ; Pecze, László

In: PLOS ONE, 2017, vol. 12, no. 6, p. e0179950

There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction of acute pain and neurological inflammation. MRS1477, a dihydropyridine derivative acts as a positive...

Université de Fribourg

Endogenous TRPV1 stimulation leads to the activation of the inositol phospholipid pathway necessary for sustained Ca2+ oscillations

Pecze, László ; Blum, Walter ; Henzi, Thomas ; Schwaller, Beat

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2016, vol. 1863, no. 12, p. 2905–2915

Sensory neuron subpopulations as well as breast and prostate cancer cells express functional transient receptor potential vanilloid type 1 (TRPV1) ion channels; however little is known how TRPV1 activation leads to biological responses. Agonist-induced activation of TRPV1 resulted in specific spatiotemporal patterns of cytoplasmic Ca²⁺ signals in breast and prostate cancer-derived cells....

Université de Fribourg

Prenatal valproate exposure differentially affects parvalbumin-expressing neurons and related circuits in the cortex and striatum of mice

Lauber, Emanuel ; Filice, Federica ; Schwaller, Beat

In: Frontiers in Molecular Neuroscience, 2016, vol. 9, p. -

Autism spectrum disorders (ASD) comprise a number of heterogeneous neurodevelopmental diseases characterized by core behavioral symptoms in the domains of social interaction, language/communication and repetitive or stereotyped patterns of behavior. In utero exposure to valproic acid (VPA) has evolved as a highly recognized rodent ASD model due to the robust behavioral phenotype observed in...

Université de Fribourg

Activation of endogenous TRPV1 fails to induce overstimulation-based cytotoxicity in breast and prostate cancer cells but not in pain-sensing neurons

Pecze, László ; Jósvay, Katalin ; Blum, Walter ; Petrovics, György ; Vizler, Csaba ; Oláh, Zoltán ; Schwaller, Beat

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2016, vol. 1863, no. 8, p. 2054–2064

Vanilloids including capsaicin and resiniferatoxin are potent transient receptor potential vanilloid type 1 (TRPV1) agonists. TRPV1 overstimulation selectively ablates capsaicin-sensitive sensory neurons in animal models in vivo. The cytotoxic mechanisms are based on strong Na⁺ and Ca2 + influx via TRPV1 channels, which leads to mitochondrial Ca2 + accumulation and...

Université de Fribourg

Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line

Blum, Walter ; Pecze, László ; Felley-Bosco, Emanuela ; Worthmüller-Rodriguez, Janine ; Wu, Licun ; Vrugt, Bart ; Perrot, Marc de ; Schwaller, Beat

In: In Vitro Cellular & Developmental Biology - Animal, 2015, p. 1–8

Mesothelial cells are susceptible to 10:38 05.06.2015asbestos fiber-induced cytotoxicity and on longer time scales to transformation; the resulting mesothelioma is a highly aggressive neoplasm that is considered as incurable at the present time Zucali et al. (Cancer Treatment Reviews 37:543–558, 2011). Only few murine cell culture models of immortalized mesothelial cells and mesothelioma...

Université de Fribourg

Characterization and modeling of Ca2 + oscillations in mouse primary mesothelial cells

Pecze, László ; Schwaller, Beat

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2015, vol. 1853, no. 3, p. 632–645

Brief changes in the cytosolic and intra-organellar Ca2 + concentration serve as specific signals for various physiological processes. In mesothelial cells lining the surface of internal organs and the walls of body cavities, a re-entry in the cell cycle (G₀–G₁ transition) evoked by serum re-administration induces long-lasting Ca2 + oscillations with a slowly...

Université de Fribourg

Evaluation of the toxicity of graphene derivatives on cells of the lung luminal surface

Horváth, Lenke ; Magrez, Arnaud ; Burghard, Marko ; Kern, Klaus ; Forró, László ; Schwaller, Beat

In: Carbon, 2013, vol. 64, p. 45–60

Graphene-based nanomaterials are expected to have a profound impact on a broad range of applications. However, studies devoted to investigating the putative adverse health effects of these nanomaterials are hugely underrepresented in the current scientific literature. We have investigated the in vitro short-term cellular toxicity associated with graphene derivatives (GD): graphene oxide and...

Université de Fribourg

Mechanism of capsaicin receptor TRPV1-mediated toxicity in pain-sensing neurons focusing on the effects of Na⁺/Ca2 + fluxes and the Ca2 +-binding protein calretinin

Pecze, László ; Blum, Walter ; Schwaller, Beat

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2013, vol. 1833, no. 7, p. 1680–1691

Transient receptor potential vanilloid subtype 1 (TRPV1) receptor is a pain-sensing, ligand-gated, non-selective cation channel expressed in peripheral sensory neurons. Prolonged activation of TRPV1 by capsaicin leads to cell swelling and formation of membrane blebs in rat dorsal root ganglion (DRG) neurons. Similar results were obtained in NIH3T3 fibroblast cells stably expressing TRPV1. Here,...