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Université de Fribourg

Carbon nanodots: Opportunities and limitations to study their biodistribution at the human lung epithelial tissue barrier

Durantie, Estelle ; Barosova, Hana ; Drasler, Barbara ; Rodriguez-Lorenzo, Laura ; Urban, Dominic A. ; Vanhecke, Dimitri ; Septiadi, Dedy ; Hirschi-Ackermann, Liliane ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Biointerphases, 2018, vol. 13, no. 6, p. 06D404

Inhalation of combustion-derived ultrafine particles (≤0.1 μm) has been found to be associated with pulmonary and cardiovascular diseases. However, correlation of the physicochemical properties of carbon-based particles such as surface charge and agglomeration state with adverse health effects has not yet been established, mainly due to limitations related to the detection of carbon...

Université de Fribourg

Aerosol delivery of functionalized gold nanoparticles target and activate dendritic cells in a 3D lung cellular model

Fytianos, Kleanthis ; Chortarea, Savvina ; Rodriguez-Lorenzo, Laura ; Blank, Fabian ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, and Barbara

In: ACS Nano, 2017, vol. 11, no. 1, p. 375–383

Nanocarrier design combined with pulmonary drug delivery holds great promise for the treatment of respiratory tract disorders. In particular, targeting of dendritic cells that are key immune cells to enhance or suppress an immune response in the lung is a promising approach for the treatment of allergic diseases. Fluorescently encoded poly(vinyl alcohol) (PVA)-coated gold nanoparticles,...

Université de Fribourg

Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract

Kuhn, Dagmar A. ; Hartmann, Raimo ; Fytianos, Kleanthis ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Parak, Wolfgang J.

In: Science and Technology of Advanced Materials, 2015, vol. 16, no. 3, p. 034608

Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from...

Université de Fribourg

Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Fytianos, Kleanthis ; Rodriguez-Lorenzo, Laura ; Clift, Martin J.D. ; Blank, Fabian ; Vanhecke, Dimitri ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine: Nanotechnology, Biology and Medicine, 2015, vol. 11, no. 3, p. 633–644

Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical...

Université de Fribourg

Avoiding drying-artifacts in transmission electron microscopy: Characterizing the size and colloidal state of nanoparticles

Michen, Benjamin ; Geers, Christoph ; Vanhecke, Dimitri ; Endes, Carola ; Rothen-Rutishauser, Barbara ; Balog, Sandor ; Petri-Fink, Alke

In: Scientific Reports, 2015, vol. 5, p. 9793

Standard transmission electron microscopy nanoparticle sample preparation generally requires the complete removal of the suspending liquid. Drying often introduces artifacts, which can obscure the state of the dispersion prior to drying and preclude automated image analysis typically used to obtain number-weighted particle size distribution. Here we present a straightforward protocol for...

Université de Fribourg

An in vitro testing strategy towards mimicking the inhalation of high aspect ratio nanoparticles

Endes, Carola ; Schmid, Otmar ; Kinnear, Calum ; Mueller, Silvana ; Camarero-Espinosa, Sandra ; Vanhecke, Dimitri ; Foster, E. Johan ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Weder, Christoph ; Clift, Martin J.D.

In: Particle and Fibre Toxicology, 2015, vol. 11, no. 1, p. 40

The challenge remains to reliably mimic human exposure to high aspect ratio nanoparticles (HARN) via inhalation. Sophisticated, multi-cellular in vitro models are a particular advantageous solution to this issue, especially when considering the need to provide realistic and efficient alternatives to invasive animal experimentation for HARN hazard assessment. By incorporating a systematic test-bed...

Université de Fribourg

Polyvinyl alcohol as a biocompatible alternative for the passivation of gold nanorods

Kinnear, Calum ; Burnand, David ; Clift, Martin J. D. ; Kilbinger, Andreas F. M. ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Angewandte Chemie International Edition, 2014, vol. 53, no. 46, p. 12613–12617

The functionalization of gold nanorods (GNRs) with polymers is essential for both their colloidal stability and biocompatibility. However, a bilayer of the toxic cationic surfactant cetyl trimethylammonium bromide (CTAB) adsorbed on the nanorods complicates this process. Herein, we report on a strategy for the biocompatible functionalization of GNRs with a hydrophobic polymeric precursor,...

Université de Fribourg

Dynamic depolarized light scattering of small round plasmonic nanoparticles: When imperfection is only perfect

Balog, Sandor ; Adolphe Merkle Institute, University of Fribourg, Switzerland ; Rodriguez-Lorenzo, Laura ; Monnier, Christophe A. ; Michen, Benjamin ; Obiols-Rabasa, Marc ; Casal-Dujat, Lucia ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke ; Schurtenberger, and Peter

In: The Journal of Physical Chemistry C, 2014, vol. 118, no. 31, p. 17968–17974

Although small round gold nanoparticles (Au NPs) possess only a small degree of shape anisotropy, they support localized surface plasmon resonances and exhibit intrinsic optical anisotropy. These inherent features promote depolarized light scattering, whose temporal fluctuations carry information about rotational Brownian dynamics, and thus can be used to describe the size distribution of round...

Université de Fribourg

Fluorescence-encoded gold nanoparticles: library design and modulation of cellular uptake into dendritic cells

Rodriguez-Lorenzo, Laura ; Fytianos, Kleanthis ; Blank, Fabian ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Small, 2014, vol. 10, no. 7, p. 1341–1350

In order to harness the unique properties of nanoparticles for novel clinical applications and to modulate their uptake into specific immune cells we designed a new library of homo- and hetero-functional fluorescence-encoded gold nanoparticles (Au-NPs) using different poly(vinyl alcohol) and poly(ethylene glycol)-based polymers for particle coating and stabilization. The encoded particles were...

Université de Fribourg

A comparative study of different in vitro lung cell culture systems to assess the most beneficial tool for screening the potential adverse effects of carbon nanotubes

Clift, Martin J. D. ; Endes, Carola ; Vanhecke, Dimitri ; Wick, Peter ; Gehr, Peter ; Schins, Roel P. F. ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Toxicological Sciences, 2014, vol. 137, no. 1, p. 55–64

To determine the potential inhalatory risk posed by carbon nanotubes (CNTs), a tier-based approach beginning with an in vitro assessment must be adopted. The purpose of this study therefore was to compare 4 commonly used in vitro systems of the human lung (human blood monocyte-derived macrophages [MDM] and monocyte-derived dendritic cells [MDDC], 16HBE14o- epithelial cells, and...