No exact match found for Petri-Fink Alke, using Petri Fink Alke instead...
Université de Fribourg

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

Chortarea, Savvina ; Fytianos, Kleanthis ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine, 2018, vol. 13, no. 10, p. 1169–1185

Aim: The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH2) upon repeated administration onto a 3D lung model were explored. Materials & methods: AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission ...

Université de Fribourg

Aerosol delivery of functionalized gold nanoparticles target and activate dendritic cells in a 3D lung cellular model

Fytianos, Kleanthis ; Chortarea, Savvina ; Rodriguez-Lorenzo, Laura ; Blank, Fabian ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, and Barbara

In: ACS Nano, 2017, vol. 11, no. 1, p. 375–383

Nanocarrier design combined with pulmonary drug delivery holds great promise for the treatment of respiratory tract disorders. In particular, targeting of dendritic cells that are key immune cells to enhance or suppress an immune response in the lung is a promising approach for the treatment of allergic diseases. Fluorescently encoded poly(vinyl alcohol) (PVA)-coated gold nanoparticles,...

Université de Fribourg

A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry

Clift, Martin J. D. ; Fytianos, Kleanthis ; Vanhecke, Dimitri ; Hočevar, Sandra ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Scientific Reports, 2017, vol. 7, no. 1, p. 434

Determination of the cell type specific response is essential towards understanding the cellular mechanisms associated with disease states as well as assessing cell-based targeting of effective therapeutic agents. Recently, there have been increased calls for advanced in vitro multi-cellular models that provide reliable and valuable tools correlative to in vivo. In this pursuit the ability to...

Université de Fribourg

Interaction of biomedical nanoparticles with the pulmonary immune system

Blank, Fabian ; Fytianos, Kleanthis ; Seydoux, Emilie ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara

In: Journal of Nanobiotechnology, 2017, vol. 15, p. 6

Engineered nanoparticles (NPs) offer site-specific delivery, deposition and cellular uptake due to their unique physicochemical properties and were shown to modulate immune responses. The respiratory tract with its vast surface area is an attractive target organ for innovative immunomodulatory therapeutic applications by pulmonary administration of such NPs, enabling interactions with...

Université de Fribourg

Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract

Kuhn, Dagmar A. ; Hartmann, Raimo ; Fytianos, Kleanthis ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Parak, Wolfgang J.

In: Science and Technology of Advanced Materials, 2015, vol. 16, no. 3, p. 034608

Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from...

Université de Fribourg

Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Fytianos, Kleanthis ; Rodriguez-Lorenzo, Laura ; Clift, Martin J.D. ; Blank, Fabian ; Vanhecke, Dimitri ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine: Nanotechnology, Biology and Medicine, 2015, vol. 11, no. 3, p. 633–644

Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical...

Université de Fribourg

Fluorescence-encoded gold nanoparticles: library design and modulation of cellular uptake into dendritic cells

Rodriguez-Lorenzo, Laura ; Fytianos, Kleanthis ; Blank, Fabian ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Small, 2014, vol. 10, no. 7, p. 1341–1350

In order to harness the unique properties of nanoparticles for novel clinical applications and to modulate their uptake into specific immune cells we designed a new library of homo- and hetero-functional fluorescence-encoded gold nanoparticles (Au-NPs) using different poly(vinyl alcohol) and poly(ethylene glycol)-based polymers for particle coating and stabilization. The encoded particles were...