Affiner les résultats

Incorporation of ceramides into yeast glycosylphosphatidylinositol-anchored proteins can be monitored in vitro

Bosson, Régine ; Guillas, Isabelle ; Vionnet, Christine ; Roubaty, Carole ; Conzelmann, Andreas

In: Eukaryotic Cell, 2009, vol. 8, no. 3, p. 306-314

After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, a fatty acid of the diacylglycerol moiety is exchanged for a C26:0 fatty acid through the subsequent actions of Per1 and Gup1. In most GPI anchors the thus modified diacylglycerol-based anchor is subsequently transformed into a ceramide-containing anchor, a reaction, which requires Cwh43. Here...