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Haute Ecole d'Ingénierie

Analysis of sea urchin Paracentrotus lividus microbiota

Boraiy, Logeina ; Schmid, Sergio (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2018.

The aim of this work is to identify the microbiota of Paracentrotus lividus with the 16S rDNA method and to verity if they produce biologically active molecules with antimicrobial and embryological assays.

Haute Ecole d'Ingénierie

Evaluation of activated alginate-GO beads for removal of pharmaceuticals from water : bachelor's thesis : diploma 2016

Jaquet, Yannick ; Fischer, Fabian (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2016.

The aim of this project was to produce calcium alginate beads with graphene oxide incorporated into the structure, in order to enhance the adsorption properties. The effects of initial concentration, adsorbent dose, pH, temperature, contact time were investigated. Adsorption kinetics and isotherms were evaluated with different models such as pseudo-first-order, pseudo-second-order,...

Haute Ecole d'Ingénierie

Isolation and characterization of sea urchin P.lividus microbiota from coelomic fluid : bachelor's thesis : diploma 2016

Zuber, Boris ; Schmid, Sergio (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2016.

The identification of the microbiota present in the coelomic fluid of Paracentrotus lividus has been highlighted in order to understand the role of bacteria in the physiology, ecology and aquaculture activities of this echinoderm.

Haute Ecole d'Ingénierie

Catalytic performance and enantioselectivity of engineered oxidoreductases as biocatalysts

Petruzzi, Loric ; Pfeifer, Marc (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2014.

The thesis will evaluate the catalytic activity and enantioselectivity of native Old Yellow Enzyme from S.pastoranius in comparison with three engineered enzyme variants. Substrates will be bioactive compounds with a particular emphasis on terpenoids and fragrances.

Haute Ecole d'Ingénierie

Développement de la mobilité ionique pour la détection des sites de sumoylation des protéines = Identification of SUMOylation Sites using High-Field Asymmetric Waveform Ion Mobility (FAIMS) coupled to a Linear Ion Trap/Orbitrap Mass Spectrometer

Pfammatter, Sibylle ; Kalman, Franka (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2014.

SUMOylation is a post-translational modification of proteins that is involved in different cellular processes including mitosis, DNA replication and transcription. However its detection by liquid chromatography coupled to mass spectrometry (LC-MS) can be challenging due to its low abundance and dynamic regulation. High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) helps in...

Haute Ecole d'Ingénierie

Development of an analytical method usefull for the quantification of L-Glu & GlutaMAX during fermentation

Mayor, Mathieu ; Kalman, Franka (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2013.

The objectives of this work are to establish time and cost-efficient methods for the quantification of L-Glutamine and GlutamaxTM in different mediums containing serums. These methods have to be easy to perform and based on a purpose of daily basis analysis of mammalian cells cultures.

Haute Ecole d'Ingénierie

Analyse de la structure secondaire de protéines par FTIR

Ruffieux, Bastien ; Segura, Jean-Manuel (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2012.

Development, implementation and validation of an analytical method to quantify the secondary structure of proteins in water using Fourier-transform infrared spectroscopy.

Haute Ecole d'Ingénierie

Role of the Bcl-2 family member Bok in proliferation or cell cycle control

Roh, Laetitia ; Schnyder, Bruno (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2011.

Bok is a pro-apoptotic protein which its exact role is unknown but may be a potential interest for cancer therapy. The function of Bok in proliferation or cell cycle control in cells is studied with a Bok-deficient mouse model

Haute Ecole d'Ingénierie

Quantification of biohydrogen production on defined media

Sautaux, Christel ; Kurt Eyer, Sergio Schmid (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2010.

Objectives of this bachelor’s Thesis was the quantification of biohydrogen production using Enterobacter aerogenes DSM 30053 with a defined medium.

Haute Ecole d'Ingénierie

Enhancing product quantity by controlling the specific growth rate of Pichia pastoris expressing human serum albumin

Hama, Adel ; Eyer, Kurt (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2010.

Establish a fed-batch process to culture a Pichia pastoris strain producing a recombinant protein (hSA), highlighting the relationship between the specific growth rate and the protein quality, as well as seeking for enhanced productivity.