In: Metallomics, 2012, vol. 4, no. 3, p. 253-259
The HEW lysozyme (Lys) and the fac-[Re(CO)3(H2O)3]+ complex (1) are used as a simple model system for the description of a new approach to the labelling polypeptides with fluorescent tags. The strategy takes advantage of the reaction of an acridine orange-based fluorophore (AO) with the non-native metal fragment 1 hybridized on the enzyme. A synthetic methodology for the quantitative...
|
In: Dalton Transactions, 2016, vol. 45, no. 4, p. 1504–1513
Mixed CO/NO-releasing molecules were prepared by conjugation of the 17-electron rhenium dicarbonyl cis-[Re(CO)₂Br₄]²⁻ complex to N-nitrosamine modified cyanocobalamin (B12) bio-vectors. The species were fully characterized by standard analytical techniques, including X-ray crystallography for cyanocobalamin-5′-O-pyrazine and (1) and its...
|
In: Dalton Transactions, 2015, vol. 44, no. 15, p. 6999–7008
Constitutional isomers of cyanocobalamin adducts based on a fluorescent rhenium tris-carbonyl diimine complex were prepared, characterized and tested against PC-3 cancer cells. The adducts differ only in the relative binding position of the organometallic species which is either bound at the cyano or the 5′-hydroxo group of vitamin B₁₂. When tested for their cytotoxic potency, the species...
|
