Université de Fribourg

Somatic calcium level reports integrated spiking activity of cerebellar interneurons in vitro and in vivo

Franconville, Romain ; Revet, Gaëlle ; Astorga, Guadalupe ; Schwaller, Beat ; Llano, Isabel

In: Journal of Neurophysiology, 2011, vol. 106, no. 4, p. 1793-1805

We examined the relationship between somatic Ca²⁺ signals and spiking activity of cerebellar molecular layer interneurons (MLIs) in adult mice. Using two-photon microscopy in conjunction with cell-attached recordings in slices, we show that in tonically firing MLIs loaded with high-affinity Ca²⁺ probes, Ca²⁺-dependent fluorescence transients are absent. Spike-triggered averages of...

Université de Fribourg

Engrailed-2 regulates genes related to vesicle formation and transport in cerebellar Purkinje cells

Holst, Martin I. ; Maercker, Christian ; Pintea, Bogdan ; Masseroli, Marco ; Liebig, Christian ; Jankowski, Jakob ; Miething, Andreas ; Martini, Julia ; Schwaller, Beat ; Oberdick, John ; Schilling, Karl ; Baader, Stephan L.

In: Molecular and Cellular Neuroscience, 2008/38/4/10.1016/j.mcn.2008.04.010

Engrailed transcription factors regulate survival, cell fate decisions and axon pathfinding in central neurons. En-2 can also attenuate Purkinje cell (PC) maturation. Here, we use array analysis to scrutinize gene expression in developing PCs overexpressing Engrailed-2 (L7En-2). The majority (70%) of regulated genes was found down-regulated in L7En-2 cerebella, consistent with the known...

Université de Fribourg

Developmental changes in Parvalbumin regulate presynaptic Ca²⁺ signaling

Collin, Thibault ; Chat, Mireille ; Lucas, Marie Gabrielle ; Moreno, Herman ; Racay, Peter ; Schwaller, Beat ; Marty, Alain ; Llano, Isabel

In: The Journal of Neuroscience, 2005, vol. 25(1), p. 96

Certain interneurons contain large concentrations of specific Ca²⁺-binding proteins (CBPs), but consequences on presynaptic Ca²⁺ signaling are poorly understood. Here we show that expression of the slow CBP parvalbumin (PV) in cerebellar interneurons is cell specific and developmentally regulated, leading to characteristic changes in presynaptic Ca²⁺ dynamics (Cai). Using...