In: Scientific Reports, 2020, vol. 10, no. 1, p. 6072
Cell migration involves dynamic changes in cell shape. Intricate patterns of cell shape can be analyzed and classified using advanced shape descriptors, including spherical harmonics (SPHARM). Though SPHARM have been used to analyze and classify migrating cells, such classification did not exploit SPHARM spectra in their dynamics. Here, we examine whether additional information from dynamic...
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In: Science Immunology, 2020, vol. 5, no. 46, p. eaaz4371
It is well established that tissue macrophages and tissue-resident memory CD8+ T cells (TRM) play important roles for pathogen sensing and rapid protection of barrier tissues. In contrast, the mechanisms by which these two cell types cooperate for homeostatic organ surveillance after clearance of infections is poorly understood. Here, we used intravital imaging to show that TRM dynamically...
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In: The Journal of Immunology, 2019, p. -
Flotillin-1 (Flot1) is an evolutionary conserved, ubiquitously expressed lipid raft– associated scaffolding protein. Migration of Flot1-deficient neutrophils is impaired because of a decrease in myosin II–mediated contractility. Flot1 also accumulates in the uropod of polarized T cells, suggesting an analogous role in T cell migration. In this study, we analyzed morphology and migration...
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In: Immunological Reviews, 2019, vol. 289, no. 1, p. 232–249
CD8+ T cells have evolved as one of the most motile mammalian cell types, designed to continuously scan peptide–major histocompatibility complexes class I on the surfaces of other cells. Chemoattractants and adhesion molecules direct CD8+ T‐cell homing to and migration within secondary lymphoid organs, where these cells colocalize with antigen‐presenting dendritic cells in confined...
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