Université de Fribourg

Acquisition of extended-spectrum β-lactamase GES-6 leading to resistance to ceftolozane-tazobactam combination in Pseudomonas aeruginosa

Poirel, Laurent ; Ortiz De La Rosa, José-Manuel ; Kieffer, Nicolas ; Dubois, Véronique ; Jayol, Aurélie ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 1, p. e01809-18

A clinical Pseudomonas aeruginosa isolate resistant to all β-lactams, including ceftolozane-tazobactam and carbapenems, was recovered. It belonged to sequence type 235 and produced the extended-spectrum β-lactamase (ESBL) GES-6 differing from GES-1 by two amino acid substitutions (E104K and G170S). GES-6 possessed an increased hydrolytic activity toward carbapenems and to ceftolozane and a ...

Université de Fribourg

Performances of the rapid polymyxin Acinetobacter and Pseudomonas tests for colistin susceptibility testing

Lescat, Mathilde ; Poirel, Laurent ; Jayol, Aurélie ; Nordmann, Patrice

In: Microbial Drug Resistance, 2018, p. -

Owing to the emergence of colistin resistance in nonfermenting Gram negative bacteria, reliable and rapid techniques for testing colistin susceptibility are needed. We evaluated the performances of the Rapid Polymyxin Acinetobacter and Pseudomonas tests using a collection of Acinetobacter baumannii and Pseudomonas aeruginosa clinical isolates.Methods: Colistin susceptibility of A. baumannii...

Université de Fribourg

Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

Jayol, Aurélie ; Kieffer, Nicolas ; Poirel, Laurent ; Guérind, François ; Güneser, Deniz ; Cattoir, Vincent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 90–94

The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin- susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3...

Université de Fribourg

Detection of colistin-resistant Gram-negative rods by using the SuperPolymyxin medium

Jayol, Aurélie ; Poirel, Laurent ; André, Catherine ; Dubois, Véronique ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 95–101

The commercial SuperPolymyxin medium (ELITechGroup, Puteaux, France), currently licensed in Europe, is aimed to screen for either intrinsic or acquired colistin resistance in Gram negatives. It was tested for screening colistin-resistant Gram- negative rods from bacterial cultures of 145 colistin-resistant and 86 colistin- susceptible Gram-negative rods isolates. It exhibited a sensitivity and...

Université de Fribourg

A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria

Nordmann, Patrice ; Mazé, Alain ; Culebras, Esther ; Dobias, Jan ; Jayol, Aurélie ; Poirel, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 91, no. 2, p. 118–122

The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20)....

Université de Fribourg

Evaluation of three broth microdilution systems to determine colistin susceptibility of Gram-negative bacilli

Jayol, Aurélie ; Nordmann, Patrice ; André, Catherine ; Poirel, Laurent ; Dubois, Véronique

In: Journal of Antimicrobial Chemotherapy, 2018, p. -

The broth microdilution (BMD) method is currently the recommended technique to determine susceptibility to colistin.Objectives: We evaluated the accuracy of three commercialized BMD panels [Sensititre (ThermoFisher Diagnostics), UMIC (Biocentric) and MicroScan (Beckman Coulter)] to determine colistin susceptibility.Methods: A collection of 185 isolates of Gram-negative bacilli (133 colistin...

Université de Fribourg

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Jayol, Aurélie ; Corlouer, Camille ; Haenni, Marisa ; Darty, Mélanie ; Maillard, Karine ; Desroches, Marine ; Lamy, Brigitte ; Jumas-Bilak, Estelle ; Madec, Jean-Yves ; Decousser, Jean-Winoc

In: European Journal of Clinical Microbiology & Infectious Diseases, 2018, p. 1–7

Stenotrophomonas maltophilia (Sm) is an archetypal environmental opportunistic bacterium responsible for health care-associated infections. The role of animals in human Sm infections is unknown. This study aims to reveal the genetic and phylogenetic relationships between pathogenic strains of Sm, both animal and human, and identify a putative role for animals as a reservoir in human...

Université de Fribourg

Prevalence of faecal carriage of colistin-resistant Gram-negative rods in a university hospital in western France, 2016

Saly, Marion ; Jayol, Aurélie ; Poirel, Laurent ; Megraud, Francis ; Nordmann, Patrice ; Dubois, Véronique

In: Journal of Medical Microbiology, 2017, vol. 66, no. 6, p. 842–843

Plasmid-mediated and chromosomally-encoded colistin resistance is increasingly being reported worldwide. We aimed to determine the prevalence of faecal carriage of colistin-resistant Gram-negative rod isolates in a university hospital in western France. From February to May 2016, rectal swabs from 653 patients hospitalized in various clinical settings were recovered and subsequently screened...