Université de Fribourg

Endothelial nitric oxide synthase gene transfer inhibits human smooth muscle cell migration via inhibition of Rho A

Largiadèr, Thomas ; Eto, Masato ; Payeli, Sravan K. ; Greutert, Helen ; Viswambharan, Hema ; Lachat, Mario ; Zünd, Gregor ; Yang, Zhihong ; Tanner, Felix C. ; Lüscher, Thomas F.

In: Journal of Cardiovascular Pharmacology, 2008, vol. 52, no. 4, p. 369-374

Smooth muscle cell (SMC) migration contributes to vascular remodeling. Nitric oxide (NO) produced via endothelial NO synthase (eNOS) inhibits SMC migration. This study analyzes signal transduction mechanisms of SMC migration targeted by NO. SMCs were cultured from human saphenous veins, and cell migration was studied using Boyden chambers. PDGF-BB (0.1 to 10 ng/ml) stimulated SMC migration in a...

Université de Fribourg

Statins prevent pulsatile stretch-induced proliferation of human saphenous vein smooth muscle cells via inhibition of Rho/Rho-kinase pathway

Kozai, Toshiyuki ; Eto, Masato ; Yang, Zhihong ; Shimokawa, Hiroaki ; Lüscher, Thomas F.

In: Cardiovascular Research, 2005, vol. 68, no. 3, p. 475-482

Objective: Pulsatile forces regulate vascular remodeling and trigger vascular diseases such as saphenous vein graft disease. The saphenous vein is exposed to high pressure and pulsatility only after implantation. Statins have been proved to reduce the incidence of vein graft failure. Thus, we investigated the molecular mechanisms of pulsatile stretch-induced saphenous vein smooth...