Université de Fribourg

Influenza A virus induces autophagosomal targeting of ribosomal proteins

Becker, Andrea C. ; Gannagé, Monique ; Giese, Sebastian ; Hu, Zehan ; Abou-Eid, Shadi ; Roubaty, Carole ; Paul, Petra ; Bühler, Lea ; Gretzmeier, Christine ; Dumit, Veronica I. ; Kaeser-Pebernard, Stéphanie ; Schwemmle, Martin ; Münz, Christian ; Dengjel, Jörn

In: Molecular & Cellular Proteomics, 2018, vol. 17, no. 10, p. 1909–1921

Seasonal epidemics of influenza A virus are a major cause of severe illness and are of high socio-economic relevance. For the design of effective antiviral therapies, a detailed knowledge of pathways perturbed by virus infection is critical. We performed comprehensive expression and organellar proteomics experiments to study the cellular consequences of influenza A virus infection using three...

Université de Fribourg

Role of influenza A virus NP acetylation on viral growth and replication

Giese, Sebastian ; Ciminski, Kevin ; Bolte, Hardin ; Moreira, Étori Aguiar ; Lakdawala, Seema ; Hu, Zehan ; David, Quinnlan ; Kolesnikova, Larissa ; Götz, Veronika ; Zhao, Yongxu ; Dengjel, Jörn ; Chin, Y. Eugene ; Xu, Ke ; Schwemmle, Martin

In: Nature Communications, 2017, vol. 8, no. 1, p. 1259

Lysine acetylation is a post-translational modification known to regulate protein functions. Here we identify several acetylation sites of the influenza A virus nucleoprotein (NP), including the lysine residues K77, K113 and K229. Viral growth of mutant virus encoding K229R, mimicking a non-acetylated NP lysine residue, is severely impaired compared to wildtype or the mutant viruses encoding...