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Monitoring sterol uptake, acetylation, and export in yeast

Choudhary, Vineet ; Schneiter, Roger

In: Methods in Molecular Biology, 2009, vol. 580, p. 221-232

Sterols are essential lipid components of eukaryotic membranes. They are synthesized in the endoplasmatic reticulum (ER) from where they are efficiently transported to the plasma membrane, which harbors ~90% of the free sterol pool of the cell. The molecular mechanisms that govern this lipid transport, however, are not well characterized and are challenging to analyze. Saccharomyces cerevisiae...