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Université de Fribourg

Modulation of calretinin expression in human mesothelioma cells reveals the implication of the fak and wnt signaling pathways in conferring chemoresistance towards cisplatin

Wörthmüller, Janine ; Salicio, Valérie ; Oberson, Anne ; Blum, Walter ; Schwaller, Beat

In: International Journal of Molecular Sciences, 2019, vol. 20, no. 21, p. 5391

Malignant mesothelioma (MM) is an aggressive asbestos-linked neoplasm, characterized by dysregulation of signaling pathways. Due to intrinsic or acquired chemoresistance, MM treatment options remain limited. Calretinin is a Ca2+-binding protein expressed during MM tumorigenesis that activates the FAK signaling pathway, promoting invasion and epithelial-to-mesenchymal transition. Constitutive...

Université de Fribourg

Inducible and reversible silencing of the Pvalb gene in mice: An in vitro and in vivo study

Filice, Federica ; Blum, Walter ; Lauber, Emanuel ; Schwaller, Beat

In: European Journal of Neuroscience, 2019, vol. 50, no. 4, p. 2694–2706

Inducible and reversible regulation of gene expression is a powerful approach for unraveling gene functions. Here, we describe the generation of a system to efficiently downregulate in a reversible and inducible manner the Pvalb gene coding for the calcium‐binding protein parvalbumin (PV) in mice. We made use of an IPTG‐inducible short hairpin RNA to activate Pvalb transcript knockdown...

Université de Fribourg

Parvalbumin expression in oligodendrocyte-like CG4 cells causes a reduction in mitochondrial volume, attenuation in reactive oxygen species production and a decrease in cell processes’ length and branching

Lichvarova, Lucia ; Blum, Walter ; Schwaller, Beat ; Szabolcsi, Viktoria

In: Scientific Reports, 2019, vol. 9, no. 1, p. 10603

Forebrain glial cells - ependymal cells and astrocytes -acquire upon injury- a “reactive” phenotype associated with parvalbumin (PV) upregulation. Since free radicals, e.g. reactive oxygen species (ROS) play a role in the pathogenesis of multiple sclerosis, and that PV-upregulation in glial cells is inversely correlated with the level of oxidative stress, we hypothesized that...

Université de Fribourg

Absence of calretinin protein expression in malignant mesotheliomas from asbestos-exposed NF2+/− mice and mouse mesothelioma cell lines from various mouse strains

Blum, Walter ; Henzi, Thomas ; Châtel-Soulet, Hugues-Etienne ; Pecze, Laszlo ; Wörthmüller Rodriguez, Janine ; Vrugt, Bart ; Schwaller, Beat

In: Biomarker Research, 2018, vol. 6, no. 1, p. 19

Calretinin is the most widespread positive marker for the immunohistochemical identification of malignant mesothelioma (MM) and was proposed to serve as a blood- based biomarker. Functionally, evidence has accumulated that calretinin might be implicated in MM tumorigenesis. We aimed to identify calretinin (CR; Calb2) in murine MM and reactive mesothelial cells in granuloma from...

Université de Fribourg

Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter

Blum, Walter ; Pecze, László ; Wörthmüller Rodriguez, Janine ; Steinauer, Martine ; Schwaller, Beat

In: BMC Cancer, 2018, vol. 18, p. 475

The calcium-binding protein calretinin (gene name: CALB2) is currently considered as the most sensitive and specific marker for the diagnosis of malignant mesothelioma (MM). MM is a very aggressive tumor strongly linked to asbestos exposure and with no existing cure so far. The mechanisms of calretinin regulation, as well as its distinct function in MM are still poorly understood.

Université de Fribourg

Putative cancer stem cells may be the key target to inhibit cancer cell repopulation between the intervals of chemoradiation in murine mesothelioma

Wu, Licun ; Blum, Walter ; Zhu, Chang-Qi ; Yun, Zhihong ; Pecze, Laszlo ; Kohno, Mikihiro ; Chan, Mei-Lin ; Zhao, Yidan ; Felley-Bosco, Emanuela ; Schwaller, Beat ; Perrot, Marc de

In: BMC Cancer, 2018, vol. 18, p. 471

Cancer cell repopulation during chemotherapy or radiotherapy is a major factor limiting the efficacy of treatment. Cancer stem cells (CSC) may play critical roles during this process. We aim to demonstrate the role of mesothelioma stem cells (MSC) in treatment failure and eventually to design specific target therapies against MSC to improve the efficacy of treatment in malignant mesothelioma.

Université de Fribourg

How asbestos drives the tissue towards tumors: YAP activation, macrophage and mesothelial precursor recruitment, RNA editing, and somatic mutations

Rehrauer, Hubert ; Wu, Licun ; Blum, Walter ; Pecze, Lazslo ; Henzi, Thomas ; Serre-Beinier, Véronique ; Aquino, Catherine ; Vrugt, Bart ; Perrot, Marc de ; Schwaller, Beat ; Felley-Bosco, Emanuela

In: Oncogene, 2018, vol. 37, no. 20, p. 2645–2659

Chronic exposure to intraperitoneal asbestos triggered a marked response in the mesothelium well before tumor development. Macrophages, mesothelial precursor cells, cytokines, and growth factors accumulated in the peritoneal lavage. Transcriptome profiling revealed YAP/TAZ activation in inflamed mesothelium with further activation in tumors, paralleled by increased levels of cells with...

Université de Fribourg

Biological noise and positional effects influence cell stemness

Blum, Walter ; Henzi, Thomas ; Schwaller, Beat ; Pecze, László

In: Journal of Biological Chemistry, 2018, vol. 293, no. 14, p. 5247–5258

Biological (or cellular) noise is the random quantitative variability of proteins and other molecules in individual, genetically identical cells. As the result of biological noise in the levels of some transcription factors that determine a cell's differentiation status, differentiated cells may dedifferentiate to a stem cell state given a sufficiently long time period. Here, to provide...

Université de Fribourg

Menthol evokes Ca2+ signals and induces oxidative stress independently of the presence of TRPM8 (menthol) receptor in cancer cells

Nazıroğlu, Mustafa ; Blum, Walter ; Jósvay, Katalin ; Çiğ, Bilal ; Henzi, Thomas ; Oláh, Zoltán ; Vizler, Csaba ; Schwaller, Beat ; Pecze, László

In: Redox Biology, 2018, vol. 14, p. 439–449

Menthol is a naturally occurring monoterpene alcohol possessing remarkable biological properties including antipruritic, analgesic, antiseptic, anti-inflammatory and cooling effects. Here, we examined the menthol-evoked Ca2+ signals in breast and prostate cancer cell lines. The effect of menthol (50–500µM) was predicted to be mediated by the transient receptor potential ion channel...