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Università della Svizzera italiana

Stepwise 5' DNA end-specific resection of DNA breaks by the Mre11-Rad50-Xrs2 and Sae2 nuclease ensemble

Cannavo, Elda ; Reginato, Giordano ; Cejka, Petr

In: Proceedings of the national academy of sciences of the United States of America, 2019, vol. 116, no. 12 (March 19), p. 5505-5513

To repair DNA double-strand breaks by homologous recombination, the 5′-terminated DNA strands must first be resected to produce 3′ overhangs. Mre11 from Saccharomyces cerevisiae is a 3′ → 5′ exonuclease that is responsible for 5′ end degradation in vivo. Using plasmid-length DNA substrates and purified recombinant proteins, we show that the combined exonuclease and endonuclease...