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Université de Fribourg

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

Abou-Mansour, Eliane ; Débieux, Jean-Luc ; Ramírez-Suero, Montserrat ; Bénard-Gellon, Mélanie ; Magnin-Robert, Maryline ; Spagnolo, Alessandro ; Chong, Julie ; Farine, Sibylle ; Bertsch, Christohpe ; L’Haridon, Floriane ; Serrano, Mario ; Fontaine, Florence ; Rego, Cecilia ; Larignon, Philippe

In: Phytochemistry, 2015, vol. 115, p. 207–215

Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic...

Université de Fribourg

Differential responses of three grapevine cultivars to Botryosphaeria dieback

Spagnolo, Alessandro ; Magnin-Robert, Maryline ; Alayi, Tchilabalo Dilezitoko ; Cilindre, Clara ; Schaeffer-Reiss, Christine ; Dorsselaer, Alain Van ; Clément, Christophe ; Larignon, Philippe ; Suero-Ramirez, Monserrat ; Chong, Julie ; Bertsch, Christophe ; Abou-Mansour, Eliane ; Fontaine, Florence

In: Phytopathology, 2014, p. -

Botryosphaeria dieback is a fungal grapevine trunk disease which represents a threat for viticulture worldwide due to the decreased production of affected plants and their premature death. This dieback is characterized by a typical wood discoloration called “brown stripe”. Herein, a proteome comparison of the brown striped wood from Botryosphaeria dieback-affected standing vines cultivar...

Université de Fribourg

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Ramírez-Suero, M. ; Bénard-Gellon, M. ; Chong, J. ; Laloue, H. ; Stempien, E. ; Abou-Mansour, Eliane ; Fontaine, F. ; Larignon, Philippe ; Mazet-Kieffer, F. ; Farine, S. ; Bertsch, C.

In: Protoplasma, 2014, p. 1–10

Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for...

Université de Fribourg

Grapevine trunk diseases: complex and still poorly understood

Bertsch, C. ; Ramírez-Suero, M. ; Magnin-Robert, M. ; Larignon, Philippe ; Chong, J. ; Abou-Mansour, Eliane ; Spagnolo, A. ; Clément, C. ; Fontaine, F.

In: Plant Pathology, 2013, vol. 62, no. 2, p. 243–265

This review presents an overview of eutypa dieback, esca and botryosphaeria dieback, the predominant grapevine trunk diseases worldwide. It covers their symptomatologies in the trunk, leaves and berries; the characteristics of the different fungal species associated with them; and host–pathogen interactions. Here, the host–pathogen relationship is defined at the cytological, physiological and...

Université de Fribourg

Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine

Djoukeng, Jules Désiré ; Polli, Suzanna ; Larignon, Philippe ; Abou-Mansour, Eliane

In: European Journal of Plant Pathology, 2009, vol. 124, no. 2, p. 303-308

A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7- hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ -resveratrol into the...