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Université de Fribourg

Genetic evidence that expression of NahG modifies defence pathways independent of salicylic acid biosynthesis in the Arabidopsis–Pseudomonas syringae pv. tomato interaction

Heck, Silvia ; Grau, Theres ; Buchala, Antony ; Métraux, Jean-Pierre ; Nawrath, Christiane

In: The Plant Journal, 2003, vol. 36(3), p. 342

The salicylic acid (SA)-induction deficient (sid) mutants of Arabidopsis, eds5 and sid2 accumulate normal amounts of camalexin after inoculation with Pseudomonas syringae pv. tomato (Pst), while transgenic NahG plants expressing an SA hydroxylase that degrades SA have reduced levels of camalexin and exhibit a higher susceptibility to different...

Université de Fribourg

Characterization of Arabidopsis enhanced disease susceptibility mutants that are affected in systemically induced resistance

Ton, Jurriaan ; Vos, Martin De ; Robben, Charlotte ; Buchala, Anthony ; Métraux, Jean-Pierre ; Loon, L. C. Van ; Pieterse, Corné M. J.

In: The Plant Journal, 2002, vol. 29(1), p. 11

In Arabidopsis, the rhizobacterial strain Pseudomonas fluorescens WCS417r triggers jasmonate (JA)- and ethylene (ET)-dependent induced systemic resistance (ISR) that is effective against different pathogens. Arabidopsis genotypes unable to express rhizobacteria-mediated ISR against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000)...

Université de Neuchâtel

Vacuolar system distribution in Arabidopsis tissues, visualized using GFP fusion proteins

Flückiger, Ricardo ; De Caroli, Monica ; Piro, Gabriella ; Dalessandro, Giuseppe ; Neuhaus, Jean-Marc ; Di Sansebastiano, Gian-Pietro

In: Journal of Experimental Botany, 2003, vol. 54, p. 1577-1584

Green fluorescent protein (GFP) allows the direct visualization of gene expression and the subcellular localization of fusion proteins in living cells. The localization of different GFP fusion proteins in the secretory system was studied in stably transformed Arabidopsis plants cv. Wassilewskaja. Secreted GFP (SGFP) and GFP retained in the ER (GFP-KDEL) confirmed patterns already known, but two...

Université de Fribourg

Isolation and characterization of signal transduction mutants of Arabidopsis thaliana that constitutively activate the octadecanoid pathway and form necrotic microlesions

Hilpert, Bernadette ; Bohlmann, Holger ; Camp, Roel op den ; Przybyla, Dominika ; Miersch, Otto ; Buchala, Anthony ; Apel, Klaus

In: The Plant Journal, 2001, vol. 26, p. 435

Thionins are a group of antimicrobial polypeptides that form part of the plant's defense mechanism against pathogens. The Thi 2.1 thionin gene of Arabidopsis thaliana has been shown to be inducible by jasmonic acid (JA), an oxylipin-like hormone derived from oxygenated linolenic acid and synthesized via the octadecanoid pathway. The JA-dependent regulation of the Thi 2.1 gene...

Université de Fribourg

Induction of (1→3,1→4)-ß-D-Glucan hydrolases in leaves of dark-incubated barley seedlings

Roulin, Samuel ; Buchala, Antony J. ; Fincher, Geoffrey B.

In: Planta, 2002, vol. 215(1), p. 51 - 59

When seedlings of barley (Hordeum vulgare L.) were transferred from a natural light/dark cycle into darkness, (1M3,1M4)-#-D-glucan endohydrolase (EC activity in leaf extracts increased 3- to 4-fold after 2 days. Activity decreased to normal levels within a day if the light/dark cycle was restored. Although there are two (1M3,1M4)-#-D-glucan endohydrolase...

Université de Fribourg

NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol

Spoel, Steven H. ; Koornneef, Annemart ; Claessens, Susanne M. C. ; Korzelius, Jerôme P. ; Pelt, Johan A. van ; Mueller, Martin J. ; Buchala, Antony J. ; Métraux, Jean-Pierre ; Brown, Rebecca ; Kazan, Kemal ; Loon, L. C. van ; Dong, Xinnian ; Pieterse, Corné M. J.

In: The Plant Cell, 2003, vol. 15, p. 760

Plant defenses against pathogens and insects are regulated differentially by cross-communicating signal transduction pathways in which salicylic acid (SA) and jasmonic acid (JA) play key roles. In this study, we investigated the molecular mechanism of the antagonistic effect of SA on JA signaling. Arabidopsis plants unable to accumulate SA produced 25-fold higher levels of JA and showed enhanced...

Université de Fribourg

Phytochrome signalling modulates the SA-perceptive pathway in Arabidopsis

Genoud, Thierry ; Buchala, Antony J. ; Chua, Nam-Hai ; Métraux, Jean-Pierre

In: The Plant Journal, 2002, vol. 31(1), p. 87

The interaction of phytochrome signalling with the SA signal transduction pathway has been investigated in Arabidopsis using single and multiple mutants affected in light perception (phyA and phyB deficient) and light-signal processing (psi2, phytochrome signalling). The induction of PR1 by SA and functional analogues has been found to strictly correlate with the activity of...

Université de Fribourg

Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis requires sensitivity to jasmonate and ethylene but is not accompanied by an increase in their production

Pieterse, Corné M. J. ; Pelt, Johan A. van ; Ton, Jurriaan ; Parchmann, Stefanie ; Mueller, Martin J. ; Buchala, Antony J. ; Métraux, Jean-Pierre ; Loon, Leendert C. van

In: Physiological and Molecular Plant Pathology, 2000, vol. 57(3), p. 123

Plants develop an enhanced defensive capacity against a broad spectrum of plant pathogens after colonization of the roots by selected strains of nonpathogenic biocontrol bacteria. In Arabidopsis thaliana, this induced systemic resistance (ISR) functions independently of salicylic acid but requires an intact response to the plant hormones jasmonic acid (JA) and ethylene. To further...

Université de Fribourg

Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions

Sieber, Patrick ; Schorderet, Martine ; Ryser, Ulrich ; Buchala, Antony ; Kolattukudy, Pappachan ; Métraux, Jean-Pierre ; Nawrath, Christiane

In: Plant Cell, 2000, vol. 12, p. 721

A major structural component of the cuticle of plants is cutin. Analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. Therefore, transgenic Arabidopsis plants were generated that express and secrete a cutinase from Fusarium solani f sp pisi. Arabidopsis plants expressing the cutinase in the extracellular...

Université de Fribourg

Asymmetric μ2-1,1-azido bridged copper(II) complex: Synthesis, X-ray structure, magnetic study and DFT calculations

Zbiri, Mohamed ; Saha, Sandip ; Adhikary, Chandan ; Chaudhuri, Siddhartha ; Daul, Claude A. ; Koner, Subratanath

In: Inorganica Chimica Acta, 2006, vol. 359, p. 1193

A new rare variety asymmetric μ2-1,1-azido bridged copper(II) complex has been synthesized and characterized structurally and magnetically. The complex [Cu2L2(μ2-1,1-N3)2] · H2O · CH3OH (L = 1-(N-ortho-hydroxyacetophenimine)-2-aminoethane) (1), crystallizes in monoclinic space group, P21/n, with a = 9.469(4) Angstrom capital A, ring, b = 12.526(8) Angstrom capital A, ring, c = 12.899(10)...