Université de Fribourg

Separation and identification of mouse brain tissue microproteins using top‐down method with high resolution nanocapillary liquid chromatography mass spectrometry

Li, Wenxue ; Petruzziello, Filomena ; Zhao, Nan ; Zhao, Huiyuan ; Ye, Xueting ; Zhang, Xiaozhe ; Rainer, Gregor

In: PROTEOMICS, 2017, vol. 17, no. 12, p. -

Microproteins and endogenous peptides in the brain contain important substances that have critical roles in diverse biological processes, contributing to signal transduction and intercellular signaling. However, variability in their physical or chemical characteristics, such as molecule size, hydrophobicity, and charge states, complicate the simultaneous analysis of these compounds, although...

Université de Fribourg

High-efficiency recognition and identification of disulfide bonded peptides in rat neuropeptidome using targeted electron transfer dissociation tandem mass spectrometry

Yu, Xi ; Khani, Abbas ; Ye, Xueting ; Petruzziello, Filomena ; Gao, Huiyuan ; Zhang, Xiaozhe ; Rainer, Gregor

In: Analytical Chemistry, 2015, vol. 87, no. 23, p. 11646–11651

The main goal of the present study is to develop a method to recognize and identify endogenous intrachain disulfide bonded peptide, which are rarely sequenced in current peptidomics studies. In order to achieve highly efficient detection of these peptides in a neuropeptidome analysis, we alkylated the peptides, mined the raw mass spectrometry data, and then recognized the candidates of untreated...

Université de Fribourg

Chronic nicotine treatment impacts the regulation of opioid and non-opioid peptides in the rat dorsal striatum

Petruzziello, Filomena ; Falasca, Sara ; Andren, Per E. ; Rainer, Gregor ; Zhang, Xiaozhe

In: Molecular and Cellular Proteomics, 2013, p. -

The chronic use of nicotine, the main psychoactive ingredient of tobacco smoking, alters diverse physiological processes and consequently generates physical dependence. To understand the impact of chronic nicotine on neuropeptides, which are potential molecules associated with dependence, we conducted qualitative and quantitative neuropeptidomics on the rat dorsal striatum (DS), an important...

Université de Fribourg

Neuropeptide alterations in the tree shrew hypothalamus during volatile anesthesia

Fouillen, Laetitia ; Petruzziello, Filomena ; Veit, Julia ; Bhattacharyya, Anwesha ; Kretz, Robert ; Rainer, Gregor ; Zhang, Xiaozhe

In: Journal of Proteomics, 2012, p. -

Neuropeptides are critical signaling molecules, involved in the regulation of diverse physiological processes including energy metabolism, pain perception and brain cognitive state. Prolonged general anesthesia has an impact on many of these processes, but the regulation of peptides by general anesthetics is poorly understood. In this study, we present an in-depth characterization of the...

Université de Fribourg

High identification rates of endogenous neuropeptides from mouse brain

Zhang, Xiaozhe ; Petruzziello, Filomena ; Zani, Fabio ; Fouillen, Laetitia ; Andren, Per E. ; Solinas, Giovanni ; Rainer, Gregor

In: Journal of Proteome Research, 2012, vol. 11, no. 5, p. 2819–2827

Mass spectrometry-based neuropeptidomics is one of the most powerful approaches for identification of endogenous neuropeptides in the brain. Until now, however, the identification rate of neuropeptides in neuropeptidomics is relatively low and this severely restricts insights into their biological function. In the present study, we developed a high accuracy mass spectrometry-based approach to...

Université de Fribourg

Broad characterization of endogenous peptides in the tree shrew visual system

Ranc, Vaclav ; Petruzziello, Filomena ; Kretz, Robert ; Argandoña, Enrike G. ; Zhang, Xiaozhe ; Rainer, Gregor

In: Journal of Proteomics, 2012, vol. 75, no. 9, p. 2526–2535

Endogenous neuropeptides, acting as neurotransmitters or hormones in the brain, carry out important functions including neural plasticity, metabolism and angiogenesis. Previous neuropeptide studies have focused on peptide-rich brain regions such as the striatum or hypothalamus. Here we present an investigation of peptides in the visual system, composed of brain regions that are generally less...

Université de Fribourg

Analysis of multiple quaternary ammonium compounds in the brain using tandem capillary column separation and high resolution mass spectrometric detection

Falasca, Sara ; Petruzziello, Filomena ; Kretz, Robert ; Rainer, Gregor ; Zhang, Xiaozhe

In: Journal of Chromatography A, 2012, p. -

Endogenous quaternary ammonium compounds are involved in various physiological processes in the central nervous system. In the present study, eleven quaternary ammonium compounds, including acetylcholine, choline, carnitine, acetylcarnitine and seven other acylcarnitines of low polarity, were analyzed from brain extracts using a two dimension capillary liquid chromatography–Fourier transform...

Université de Fribourg

Extensive characterization of Tupaia belangeri Neuropeptidome using an integrated Mass Spectrometry Approach

Petruzziello, Filomena ; Fouillen, Laetitia ; Wadenstein, Henrik ; Kretz, Robert ; Andren, Per E. ; Rainer, Gregor ; Zhang, Xiaozhe

In: Journal of proteome research, 2012, vol. 11, no. 2, p. 886-896

Neuropeptidomics is used to characterize endogenous peptides in the brain of tree shrews (Tupaia belangeri). Tree shrews are small animals similar to rodents in size but close relatives of primates, and are excellent models for brain research. Currently, tree shrews have no complete proteome information available on which direct database search can be allowed for neuropeptide identification....

Université de Fribourg

Extensive characterization of Tupaia belangeri neuropeptidome using an integrated mass spectrometric approach

Petruzziello, Filomena ; Fouillen, Laetitia ; Wadensten, Henrik ; Kretz, Robert ; Andren, Per E. ; Rainer, Gregor ; Zhang, Xiaozhe

In: Journal of Proteome Research, 2012, p. -

Neuropeptidomics is used to characterize endogenous peptides in the brain of tree shrews (Tupaia belangeri). Tree shrews are small animals similar to rodents in size but close relatives of primates, and are excellent models for brain research. Currently, tree shrews have no complete proteome information available on which direct database search can be allowed for neuropeptide identification. To...