Université de Fribourg

Visual thalamocortical circuits in parvalbumin-deficient mice

Lintas, Alessandra ; Schwaller, Beat ; Villa, Alessandro E.P.

In: Brain Research, 2013, vol. 1536, p. 107–118

The dorsal lateral geniculate nucleus (dLGN) is considered as the visual gateway to the visual cortex (VC) and sends collaterals to the thalamic reticular nucleus (RTN) that in turn receives collaterals of the corticofugal feedback projections. At all levels of this thalamocortical circuit there are GABAergic neurons expressing the calcium-buffer parvalbumin (PV). The present study reports for...

Université de Fribourg

The calcium-binding protein parvalbumin modulates the firing 1 properties of the reticular thalamic nucleus bursting neurons

Albéri, Lavinia ; Lintas, Alessandra ; Kretz, Robert ; Schwaller, Beat ; Villa, Alessandro E. P.

In: Journal of Neurophysiology, 2013, vol. 109, no. 11, p. 2827–2841

The reticular thalamic nucleus (RTN) of the mouse is characterized by an overwhelming majority of GABAergic neurons receiving afferences from both the thalamus and the cerebral cortex and sending projections mainly on thalamocortical neurons. The RTN neurons express high levels of the “slow Ca²⁺ buffer” parvalbumin (PV) and are characterized by low-threshold Ca²⁺ currents,...

Université de Fribourg

Dopamine deficiency increases synchronized activity in the rat subthalamic nucleus

Lintas, Alessandra ; Silkis, Isabella G. ; Albéri, Lavinia ; Villa, Alessandro E.P.

In: Brain Research, 2012, vol. 1434, p. 142–151

Abnormal neuronal activity in the subthalamic nucleus (STN) plays a crucial role in the pathophysiology of Parkinson's disease (PD). In this study we investigated changes in rat STN neuronal activity after 28 days following the injection of 6-OHDA in the substantia nigra pars compacta (SNc). This drug provoked a lesion of SNc that induced a dopamine (DA) depletion assessed by changes in rotating...

Université de Fribourg

Simultaneous Golgi-Cox and immunofluorescence using confocal microscopy

Spiga, Saturnino ; Acquas, Elio ; Puddu, Maria C. ; Mulas, Giovanna ; Lintas, Alessandra ; Diana, Marco

In: Brain Structure and Function, 2011, p. -

Visualization of neuronal elements is of fundamental importance in modern neuroscience. Golgi-Cox impregnation is a widely employed method that provides detailed information about morphological characteristics of neurons, but none regarding their neurochemical features. Immunocytochemical procedures, on the other hand, can provide a high degree of biochemical specificity but poorer morphological...