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Characterization of Chloroplast Protein Import without Tic56, a Component of the 1-Megadalton Translocon at the Inner Envelope Membrane of Chloroplasts

Köhler, Daniel ; Montandon, Cyril ; Hause, Gerd ; Majovsky, Petra ; Kessler, Felix ; Baginsky, Sacha ; Agne, Birgit

In: Plant Physiology, 2015, vol. 167, no. 3, p. 972-990

We report on the characterization of Tic56, a unique component of the recently identified 1-MD translocon at the inner envelope membrane of chloroplasts (TIC) in Arabidopsis (Arabidopsis thaliana) comprising Tic20, Tic100, and Tic214. We isolated Tic56 by copurification with Tandem Affinity Purification-tagged Toc159 in the absence of precursor protein, indicating spontaneous and...