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Université de Fribourg

Architecture of lipid droplets in endoplasmic reticulum is determined by phospholipid intrinsic curvature

Choudhary, Vineet ; Golani, Gonen ; Joshi, Amit S. ; Cottier, Stéphanie ; Schneiter, Roger ; Prinz, William A. ; Kozlov, Michael M.

In: Current Biology, 2018, vol. 28, no. 6, p. 915-926.e9

Lipid droplets (LDs) store fats and play critical roles in lipid and energy homeostasis. They form between the leaflets of the endoplasmic reticulum (ER) membrane and consist of a neutral lipid core wrapped in a phospholipid monolayer with proteins. Two types of ER-LD architecture are thought to exist and be essential for LD functioning. Maturing LDs either emerge from the ER into the...

Université de Fribourg

The sterol-binding activity of PATHOGENESIS-RELATED PROTEIN 1 reveals the mode of action of an antimicrobial protein

Gamir, Jordi ; Darwiche, Rabih ; Hof, Pieter van't ; Choudhary, Vineet ; Stumpe, Michael ; Schneiter, Roger ; Mauch, Felix

In: The Plant Journal, 2017, vol. 89, no. 3, p. 502–509

Pathogenesis-related proteins played a pioneering role 50 years ago in the discovery of plant innate immunity as a set of proteins that accumulated upon pathogen challenge. The most abundant of these proteins, PATHOGENESIS-RELATED 1 (PR-1) encodes a small antimicrobial protein that has become, as a marker of plant immune signaling, one of the most referred to plant proteins. The biochemical...

Université de Fribourg

The caveolin-binding motif of the pathogen-related yeast protein Pry1, a member of the CAP protein superfamily, is required for in vivo export of cholesteryl acetate

Choudhary, Vineet ; Darwiche, Rabih ; Gfeller, David ; Zoete, Vincent ; Michielin, Olivier ; Schneiter, Roger

In: Journal of Lipid Research, 2014, vol. 55, no. 5, p. 883–894

Proteins belonging to the CAP superfamily are present in all kingdoms of life and have been implicated in different physiological processes. Their molecular mode of action, however, is poorly understood. Saccharomyces cerevisiae expresses three members of this superfamily, pathogen-related yeast (Pry)1, -2, and -3. We have recently shown that Pry function is required for the secretion of...

Université de Fribourg

Expression of oleosin and perilipins in yeast promote formation of lipid droplets from the endoplasmatic reticulum

Jacquier, Nicolas ; Mishra, Shirish ; Choudhary, Vineet ; Schneiter, Roger

In: Journal of Cell Science, 2013, p. -

Most cells store neutral lipids in a dedicated compartment, the lipid droplet (LD). These LDs are structurally and functionally conserved across species. In higher eukaryotes, LDs are covered by abundant scaffolding proteins, such as the oleosins in plants and perilipins (PLINs) in animal cells. S. cerevisiae, however, has no homologues of these scaffolding proteins. To analyze a possible...

Université de Fribourg

Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins

Choudhary, Vineet ; Schneiter, Roger

In: Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2012, vol. 109, no. 42, p. 16882-16887

Sterols and related membrane-perturbing agents are subject to a quality control cycle. Compounds that fail to pass this control are acetylated and secreted into the culture media, whereas lipids that pass the cycle are deacetylated and retained within the cell. Here we describe the identification of a family of conserved proteins, the Pathogen-Related Yeast (PRY) proteins, as a class of...

Université de Fribourg

Lipid droplets are functionally connected to the endoplasmic reticulum in Saccharomyces cerevisiae

Jacquier, Nicolas ; Choudhary, Vineet ; Mari, Muriel ; Toulmay, Alexandre ; Health and Human Services, Bethesda, USA ; Reggiori, Fulvio ; Schneiter, Roger

In: Journal of Cell Science, 2011, vol. 124, p. 2424-2437

Cells store metabolic energy in the form of neutral lipids that are deposited within lipid droplets (LDs). In this study, we examine the biogenesis of LDs and the transport of integral membrane proteins from the endoplasmic reticulum (ER) to newly formed LDs. In cells that lack LDs, otherwise LD-localized membrane proteins are homogenously distributed in the ER membrane. Under these conditions,...

Université de Fribourg

Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum

Hodge, Christine A. ; Choudhary, Vineet ; Wolyniak, Michael J. ; Scarcelli, John J. ; Schneiter, Roger ; Cole, Charles N.

In: Journal of Cell Science, 2010, vol. 123, p. 141-151

Cells of Saccharomyces cerevisiae lacking Apq12, a nuclear envelope (NE)-endoplasmic reticulum (ER) integral membrane protein, are defective in assembly of nuclear pore complexes (NPCs), possibly because of defects in regulating membrane fluidity. We identified BRR6, which encodes an essential integral membrane protein of the NE-ER, as a dosage suppressor of apq12δ. Cells...

Université de Fribourg

Monitoring sterol uptake, acetylation, and export in yeast

Choudhary, Vineet ; Schneiter, Roger

In: Methods in Molecular Biology, 2009, vol. 580, p. 221-232

Sterols are essential lipid components of eukaryotic membranes. They are synthesized in the endoplasmatic reticulum (ER) from where they are efficiently transported to the plasma membrane, which harbors ~90% of the free sterol pool of the cell. The molecular mechanisms that govern this lipid transport, however, are not well characterized and are challenging to analyze. Saccharomyces cerevisiae...

Université de Fribourg

Mitochondrial outer membrane proteins assist Bid in Bax-mediated lipidic pore formation

Schafer, Blanca ; Quispe, Joel ; Choudhary, Vineet ; Chipuk, Jerry E. ; Ajero, Teddy G. ; Du, Han ; Schneiter, Roger ; Kuwana, Tomomi

In: Molecular Biology of The Cell, 2009, vol. 20, no. 8, p. 2276-2285

Mitochondrial outer membrane permeabilization (MOMP) is a critical step in apoptosis and is regulated by Bcl-2 family proteins. In vitro systems using cardiolipin-containing liposomes have demonstrated the key features of MOMP induced by Bax and cleaved Bid; however, the nature of the "pores" and how they are formed remain obscure. We found that mitochondrial outer membranes contained very little...