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Université de Fribourg

Resonance raman optical activity shows unusual structural sensitivity for systems in resonance with multiple excited states: vitamin B12 case

Machalska, Ewa ; Zajac, Grzegorz ; Gruca, Anna ; Zobi, Fabio ; Baranska, Malgorzata ; Kaczor, Agnieszka

In: The Journal of Physical Chemistry Letters, 2020, vol. 11, no. 13, p. 5037–5043

In this work, cobalamins with different upper axial substituents and a cobalamin derivative with a ring modification were studied using chiroptical spectroscopies, in particular resonance Raman optical activity (RROA), to shed light on the influence of structural modifications on RROA spectra in these strongly chiral systems in resonance with multiple excited states at 532 nm excitation. We...

Université de Fribourg

Novel reference-free methods for the determination of the instrumental response of Laue-type bent crystal spectrometers

Szlachetko, Monika ; Hoszowska, Joanna ; Szlachetko, Jakub ; Dousse, Jean-Claude

In: Journal of Analytical Atomic Spectrometry, 2019, vol. 34, no. 11, p. 2325–2332

We report on novel reference-free methods to determine the instrumental resolution of transmission-type bent crystal spectrometers. The novel methods are based on the measurements of a selected X-ray line in several orders of diffraction. It is shown that the angular broadening of the spectrometer and the natural linewidth of the selected transition can be obtained directly from the novel...

Université de Fribourg

Magnetic-field uniformity in neutron electric-dipole-moment experiments

Abel, C. ; Ayres, N. J. ; Baker, T. ; Ban, G. ; Bison, G. ; Bodek, K. ; Bondar, V. ; Crawford, C. B. ; Chiu, P.-J. ; Chanel, E. ; Chowdhuri, Z. ; Daum, M. ; Dechenaux, B. ; Emmenegger, S. ; Ferraris-Bouchez, L. ; Flaux, P. ; Geltenbort, P. ; Green, K. ; Griffith, W. C. ; Grinten, M. van der ; Harris, P. G. ; Henneck, R. ; Hild, N. ; Iaydjiev, P. ; Ivanov, S. N. ; Kasprzak, M. ; Kermaidic, Y. ; Kirch, K. ; Koch, H.-C. ; Komposch, S. ; Koss, P. A. ; Kozela, A. ; Krempel, J. ; Lauss, B. ; Lefort, T. ; Lemiere, Y. ; Leredde, A. ; Mohanmurthy, P. ; Pais, D. ; Piegsa, F. M. ; Pignol, G. ; Quéméner, G. ; Rawlik, M. ; Rebreyend, D. ; Ries, D. ; Roccia, S. ; Rozpedzik, D. ; Schmidt-Wellenburg, P. ; Schnabel, A. ; Severijns, N. ; Virot, R. ; Weis, Antoine ; Wursten, E. ; Wyszynski, G. ; Zejma, J. ; Zsigmond, G.

In: Physical Review A, 2019, vol. 99, no. 4, p. 042112

Magnetic-field uniformity is of the utmost importance in experiments to measure the electric dipole moment of the neutron. A general parametrization of the magnetic field in terms of harmonic polynomial modes is proposed, going beyond the linear- gradients approximation. We review the main undesirable effects of nonuniformities: depolarization of ultracold neutrons and Larmor frequency shifts...

Università della Svizzera italiana

Crystal structure and size-dependent neutralization properties of HK20, a human monoclonal antibody binding to the highly conserved heptad repeat 1 of gp41

Sabin, Charles ; Corti, Davide ; Buzon, Victor ; Seaman, Mike S. ; Hulsik, David Lutje ; Hinz, Andreas ; Vanzetta, Fabrizia ; Agatic, Gloria ; Silacci, Chiara ; Mainetti, Lara ; Scarlatti, Gabriella ; Sallusto, Federica ; Weiss, Robin ; Lanzavecchia, Antonio ; Weissenhorn, Winfried

In: Plos pathogens, 2010, vol. 6, no. 11, p. e1001195

The human monoclonal antibody (mAb) HK20 neutralizes a broad spectrum of primary HIV-1 isolates by targeting the highly conserved heptad repeat 1 (HR1) of gp41, which is transiently exposed during HIV-1 entry. Here we present the crystal structure of the HK20 Fab in complex with a gp41 mimetic 5-Helix at 2.3 A° resolution. HK20 employs its heavy chain CDR H2 and H3 loops to bind into a...