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Université de Fribourg

Tissue-specific transcription footprinting using RNA PoI DamID (RAPID) in Caenorhabditis elegans

Gómez-Saldivar, Georgina ; Osuna-Luque, Jaime ; Semple, Jennifer I. ; Glauser, Dominique A. ; Jarriault, Sophie ; Meister, Peter

In: Genetics, 2020, vol. 216, no. 4, p. 931–945

Differential gene expression across cell types underlies development and cell physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates to the difficulty to obtain comprehensive tissue-specific gene transcription data, since available methods are still challenging to execute and/or...

Université de Fribourg

Tissue-specific isoforms of the single C. elegans Ryanodine receptor gene unc-68 control specific functions

Marques, Filipe ; Thapliyal, Saurabh ; Javer, Avelino ; Shrestha, Priyanka ; Brown, André E. X. ; Glauser, Dominique A.

In: PLOS Genetics, 2020, vol. 16, no. 10, p. e1009102

Ryanodine receptors (RyR) are essential regulators of cellular calcium homeostasis and signaling. Vertebrate genomes contain multiple RyR gene isoforms, expressed in different tissues and executing different functions. In contrast, invertebrate genomes contain a single RyR-encoding gene and it has long been proposed that different transcripts generated by alternative splicing may diversify...

Université de Fribourg

S-methyl methanethiosulfonate: promising late blight inhibitor or broad range toxin?

Joller, Charlotte ; Vrieze, Mout De ; Moradi, Aboubakr ; Fournier, Claudine ; Chinchilla, Delphine ; L’Haridon, Floriane ; Bruisson, Sebastien ; Weisskopf, Laure

In: Pathogens, 2020, vol. 9, no. 6, p. 496

(1) Background: S-methyl methanethiosulfonate (MMTS), a sulfur containing volatile organic compound produced by plants and bacterial species, has recently been described to be an efficient anti-oomycete agent with promising perspectives for the control of the devastating potato late blight disease caused by Phytophthora infestans. However, earlier work raised questions regarding the putative...

Université de Fribourg

Specific ion channels control sensory gain, sensitivity, and kinetics in a tonic thermonociceptor

Saro, Gabriella ; Lia, Andrei-Stefan ; Thapliyal, Saurabh ; Marques, Filipe ; Busch, Karl Emanuel ; Glauser, Dominique A.

In: Cell Reports, 2020, vol. 30, no. 2, p. 397-408.e4

Pain sensation and aversive behaviors entail the activation of nociceptor neurons, whose function is largely conserved across animals. The functional heterogeneity of nociceptors and ethical concerns are challenges for their study in mammalian models. Here, we investigate the function of a single type of genetically identified C. elegans thermonociceptor named FLP. Using calcium imaging in...

Université de Fribourg

Reproduction, fat metabolism, and life span: what is the connection?

Hansen, Malene ; Flatt, Thomas ; Aguilaniu, Hugo

In: Cell Metabolism, 2013, vol. 17, no. 1, p. 10–19

Reduced reproduction is associated with increased fat storage and prolonged life span in multiple organisms, but the underlying regulatory mechanisms remain poorly understood. Recent studies in several species provide evidence that reproduction, fat metabolism, and longevity are directly coupled. For instance, germline removal in the nematode Caenorhabditis elegans promotes longevity in part...