Université de Fribourg

High colonization rate and heterogeneity of ESBL- and carbapenemase-producing enterobacteriaceae isolated from gull feces in Lisbon, Portugal

Aires-de-Sousa, Marta ; Fournier, Claudine ; Lopes, Elizeth ; Lencastre, Hermínia de ; Nordmann, Patrice ; Poirel, Laurent

In: Microorganisms, 2020, vol. 8, no. 10, p. 1487

In order to evaluate whether seagulls living on the Lisbon coastline, Portugal, might be colonized and consequently represent potential spreaders of multidrug-resistant bacteria, a total of 88 gull fecal samples were screened for detection of extended- spectrum β-lactamase (ESBL)- or carbapenemase-producing Enterobacteriaceae for methicillin-resistant Staphylococcus aureus (MRSA) and for...

Université de Fribourg

Epidemiology of extended-spectrum β-lactamase-producing Enterobacteriaceae among healthcare students, at the Portuguese Red Cross Health School of Lisbon, Portugal

Fournier, Claudine ; Aires de Sousa, Marta ; Fuster Escriva, Begoña ; Sales, Leila ; Nordmanna, Patrice ; Poirel, Laurent

In: Journal of Global Antimicrobial Resistance, 2020, vol. 22, p. 733–737

Objective: The aim of the present study was to prospectively evaluate the prevalence of intestinal carriage by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among Portuguese students attending a Bachelors’ course in healthcare, and to determine the molecular features of ESBL-producing isolates.Methods: One-hundred and eleven faecal samples recovered from Portuguese...

Université de Fribourg

Epidemiology of carbapenemase-producing Klebsiella pneumoniae in northern Portugal: Predominance of KPC-2 and OXA-48

Lopes, Elizeth ; Saavedra, Maria José ; Costa, Eliana ; Lencastre, Hermíniade ; Poirel, Laurent ; Aires-de-Sousa, Marta

In: Journal of Global Antimicrobial Resistance, 2020, vol. 22, p. 349–353

Objectives: To provide, for the first time, data on the molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae clinical isolates from the northern region of Portugal (Trás-os-Montes and Alto Douro).Methods: A total of 106 carbapenemase-producing K. pneumoniae isolates recovered from clinical samples and rectal swabs between January 2018 and March 2019 were included in this...

Université de Fribourg

Occurrence of NDM-1-producing Morganella morganii and Proteus mirabilis in a single patient in Portugal: probable in vivo transfer by conjugation

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Goncalves, Maria Luísa ; Costa, Augusto ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2020, vol. 75, no. 4, p. 903–906

To decipher the genetics of acquisition of carbapenemase-encoding genes identified in two carbapenem-resistant Enterobacteriaceae recovered from a single patient in Portugal.Methods: Carbapenemase genes were searched by PCR assays and mating-out assays were performed to further characterize the plasmid support of the carbapenemase genes. Genetic characterization of the plasmid supports was...

Università della Svizzera italiana

An MVA vector expressing HIV-1 envelope under the control of a potent vaccinia virus promoter as a promising strategy in HIV/AIDS vaccine design

Pérez, Patricia ; Marín, María Q. ; Lázaro-Frías, Adrián ; Sorzano, Carlos Óscar S. ; Di Pilato, Mauro ; Gómez, Carmen E. ; Esteban, Mariano ; García-Arriaza, Juan

In: Vaccines, 2019, vol. 7, no. 4, p. 208

Highly attenuated poxviral vectors, such as modified vaccinia virus ankara (MVA), are promising vaccine candidates against several infectious diseases. One of the approaches developed to enhance the immunogenicity of poxvirus vectors is increasing the promoter strength and accelerating during infection production levels of heterologous antigens. Here, we have generated and characterized the ...

Université de Fribourg

Occurrence of CTX-M-15- and MCR-1-producing Enterobacterales in pigs in Portugal: evidence of direct links with antibiotic selective pressure

Fournier, Claudine ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Poirel, Laurent

In: International Journal of Antimicrobial Agents, 2020, vol. 55, no. 2, p. 105802

AimsTo undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics.Materials and methods: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for...

Université de Fribourg

Epidemiology of Carbapenemase-Producing Klebsiella pneumoniae in a Hospital, Portugal

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Gonçalves, Maria Luísa ; Pereira, Ana Luísa ; Nordmann, Patrice ; Poirel, Laurent

In: Emerging Infectious Diseases, 2019, vol. 25, no. 9, p. 1632–1638

We aimed to provide updated epidemiologic data on carbapenem-resistant Klebsiella pneumoniae in Portugal by characterizing all isolates (N = 46) recovered during 2013– 2018 in a 123-bed hospital in Lisbon. We identified blaKPC-3 (n = 36), blaOXA-181 (n = 9), and blaGES-5 (n = 8) carbapenemase genes and observed co-occurrence of blaKPC-3 and blaGES-5 in 7 isolates. A single GES-5–producing...

Université de Fribourg

Novel insights into the morphology of Plesiochelys bigleri from the early Kimmeridgian of Northwestern Switzerland

Raselli, Irena ; Anquetin, Jérémy

In: PLOS ONE, 2019, vol. 14, no. 5, p. e0214629

Plesiochelyidae were relatively large coastal marine turtles, which inhabited the epicontinental seas of Western Europe during the Late Jurassic. Their fossil record can be tracked in Germany, Switzerland, the United Kingdom, France, Spain and Portugal. The Jura Mountains, in northwestern Switzerland, have been the main source for the study of this group, mostly thanks to the rich and famous...