Université de Fribourg

(Strept)avidin as host for biotinylated coordination complexes: stability, chiral discrimination, and cooperativity

Loosli, Andreas ; Rusbandi, Untung Edy ; Gradinaru, Julieta ; Bernauer, Klaus ; Schläpfer, Carl-Wilhelm ; Meyer, Michel ; Mazurek, Sylwester ; Novic, Marjana ; Ward, Thomas R.

In: Inorganic Chemistry, 2006, vol. 45, no. 2, p. 660-668

Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)₂(Biot-bpy)]²⁺ (1) in either avidin or streptavidin-(strept)avidin-can be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and δ)-1 species were synthesized and incorporated in...