Affiner les résultats

Type de document

Collection spécifique

Langue

Université de Fribourg

Inhibition of p38mapk reduces adipose tissue inflammation in aging mediated by arginase-II

Huang, Ji ; Liu, Chang ; Ming, Xiu-Fen ; Yang, Zhihong

In: Pharmacology, 2020, vol. 105, no. 9–10, p. 491–504

Background: Adipose tissue inflammation occurs not only in obesity but also in aging and is mechanistically linked with age-associated diseases. Studies show that ablation of the l-arginine-metabolizing enzyme arginase-II (Arg-II) reduces adipose tissue inflammation and improves glucose tolerance in obesity. However, the role of Arg-II in aging adipose tissue inflammation is not clear....

Université de Fribourg

Genetic targeting of arginase-ii in mouse prevents renal oxidative stress and inflammation in diet-induced obesity

Huang, Ji ; Rajapakse, Angana ; Xiong, Yuyan ; Montani, Jean-Pierre ; Verrey, François ; Ming, Xiu-Fen ; Yang, Zhihong

In: Frontiers in Physiology, 2016, vol. 7, p. -

Obesity is associated with development and progression of chronic kidney disease (CKD). Recent evidence demonstrates that enhanced levels of the L- arginine:ureahydrolase, including the two isoenzymes arginase-I (Arg-I) and arginase- II (Arg-II) in vascular endothelial cells promote uncoupling of endothelial nitric oxide synthase (eNOS), leading to increased superoxide radical anion and...

Université de Fribourg

Hypoxia enhances endothelial intercellular adhesion molecule 1 protein level through upregulation of arginase type II and mitochondrial oxidative stress

Liang, Xiujie ; Arullampalam, Prakash ; Yang, Zhihong ; Ming, Xiu-Fen

In: Frontiers in Physiology, 2019, vol. 10, p. -

Hypoxia plays a crucial role in the pathogenesis of cardiovascular diseases. Mitochondrial enzyme arginase type II (Arg-II) is reported to lead to endothelial dysfunction and enhance the expression of endothelial inflammatory adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). In this study, we investigate the role of Arg-II in...

Université de Fribourg

Targeting arginase-II protects mice from high-fat-diet-induced hepatic steatosis through suppression of macrophage inflammation

Liu, Chang ; Rajapakse, Angana G. ; Riedo, Erwin ; Fellay, Benoit ; Bernhard, Marie-Claire ; Montani, Jean-Pierre ; Yang, Zhihong ; Ming, Xiu-Fen

In: Scientific Reports, 2016, vol. 6, p. 20405

Nonalcoholic fatty liver disease (NAFLD) associates with obesity and type 2 diabetes. Hypoactive AMP-activated protein kinase (AMPK), hyperactive mammalian target of rapamycin (mTOR) signaling, and macrophage-mediated inflammation are mechanistically linked to NAFLD. Studies investigating roles of arginase particularly the extrahepatic isoform arginase-II (Arg-II) in...

Université de Fribourg

Thrombin Stimulates Human Endothelial Arginase Enzymatic Activity via RhoA/ROCK Pathway

Ming, Xiu-Fen ; Barandier, Christine ; Viswambharan, Hema ; Kwak, Brenda R. ; Mach, François ; Mazzolai, Lucia ; Hayoz, Daniel ; Ruffieux, Jean ; Rusconi, Sandro ; Montani, Jean-Pierre ; Yang, Zhihong

In: Circulation, 2004, vol. 110, p. 3708-3714

Background— Arginase competes with endothelial nitric oxide synthase (eNOS) for the substrate L-arginine and decreases NO production. This study investigated regulatory mechanisms of arginase activity in endothelial cells and its role in atherosclerosis. Methods and Results— In human endothelial cells isolated from umbilical veins, thrombin concentration- and time-dependently stimulated...

Université de Fribourg

Inhibition of S6K1 accounts partially for the anti-inflammatory effects of the arginase inhibitor L-norvaline

Ming, Xiu-Fen ; Rajapakse, Angana Gupta ; Carvas, João Miguel ; Ruffieux, Jean ; Yang, Zhihong

In: BMC Cardiovascular Disorders, 2009, vol. 9, no. 12, p. -

Background: Pharmacological inhibition of endothelial arginase-II has been shown to improve endothelial nitric oxide synthase (eNOS) function and reduce atherogenesis in animal models. We investigated whether the endothelial arginase II is involved in inflammatory responses in endothelial cells. Methods: Human endothelial cells were isolated from umbilical veins and stimulated with TNFα (10...

Université de Fribourg

Opposing and uncoupling effects of mTOR and S6K1 in the regulation of endothelial tissue factor expression

Ming, Xiu-Fen ; Rajapakse, Angana Gupta ; Carvas, João Miguel ; Ruffieux, Jean ; Yang, Zhihong

In: Febs Letters, 2010, vol. 58, no. 1, p. 135-140

Rapamycin has been reported to enhance tissue factor (TF) expression. The present study investigated roles of mammalian target of rapamycin (mTOR) and its downstream S6K1 in this process. We showed here that, consistent with rapamycin, knocking-down mTOR enhanced thrombin-induced TF mRNA and protein levels, whereas silencing S6K1 mitigated up-regulation of TF protein but not TF mRNA level. The...

Université de Fribourg

Perspectives of targeting mTORC1–S6K1 in cardiovascular aging

Ming, Xiu-Fen ; Montani, Jean-Pierre ; Yang, Zhihong

In: Frontiers in Vascular Physiology, 2012, vol. 3, p. 5

The global population aging is accelerating and age-associated diseases including cardiovascular diseases become more challenging. The underlying mechanisms of aging and age-associated cardiovascular dysfunction remain elusive. There are substantial evidences demonstrating a pivotal role of the mammalian target of rapamycin complex 1 (mTORC1) and its down-stream effector S6K1 signaling in...

Université de Fribourg

Endothelial nitric oxide synthase gene transfer restores endothelium–dependent relaxations and attenuates lesion formation in carotid arteries in apolipoprotein E–deficient mice

Mujynya-Ludunge, Kathi ; Viswambharan, Hema ; Driscoll, Robert ; Ming, Xiu-Fen ; Segesser, Ludwig K. von ; Kappenberger, Lukas ; Yang, Zhihong ; Vassalli, Giuseppe

In: Basic Research in Cardiology, 2005, vol. 100(2), p. 102

Nitric oxide (NO) and monocyte chemoattractant protein–1 (MCP-1) exert partly opposing effects in vascular biology. NO plays pleiotropic vasoprotective roles including vasodilation and inhibition of platelet aggregation, smooth muscle cell proliferation, and endothelial monocyte adhesion, the last effect being mediated by MCP–1 downregulation. Early stages of arteriosclerosis are associated...