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Université de Neuchâtel

Les métaphores : du savoir de la mère au savoir médical

Lecomte Andrade, Gladys

In: Bulletin VALS-ASLA, 2006, vol. 83, no. 2, p. 107-117

Université de Neuchâtel

Cultivation-independent analysis reveals a shift in ciliate 18S rRNA gene diversity in a polycyclic aromatic hydrocarbon-polluted soil

Lara, Enrique ; Berney, Cédric ; Harms, Hauke ; Chatzinotas, Antonis

In: FEMS Microbiology Ecology, 2007, vol. 62, no. 3, p. 365-373

Using cultivation-independent methods the ciliate communities of a clay-rich soil with a 90-year record of pollution by polycyclic aromatic hydrocarbons (PAH) (4.5 g kg−1 PAH) were compared with that of a nonpolluted soil collected in its vicinity and with similar properties. A ciliate-specific set of 18S rRNA gene targeting primers was designed and used to amplify DNA extracted from...

Université de Neuchâtel

Pan-oceanic distribution of new highly diverse clades of deep-sea diplonemids

Lara, Enrique ; Moreira, David ; Vereshchaka, Alexander ; López-García, Purificación

In: Environmental Microbiology, 2008, vol. 11, no. 1, p. 47-55

Molecular rRNA gene surveys reveal a considerable diversity of microbial eukaryotes in different environments. Even within a single clade, the number of distinct phylotypes retrieved often goes beyond previous expectations. Here, we have used specific 18S rRNA PCR primers to investigate the diversity of diplonemids, a poorly known group of flagellates with only a few described species. We...

Université de Neuchâtel

Molecular comparison of cultivable protozoa from a pristine and a polycyclic aromatic hydrocarbon polluted site

Lara, Enrique ; Berney, Cédric ; Ekelund, Flemming ; Harms, Hauke ; Chatzinotas, Antonis

In: Soil Biology and Biochemistry, 2007, vol. 39, no. 1, p. 139-148

We compared the abundance and diversity of cultivable protozoa (flagellates and amoebae) in a polycyclic aromatic hydrocarbon (PAH) polluted soil and an unpolluted control, by isolating and cultivating clonal strains. The number of cultivable protozoa was higher in the polluted soil; however, the polluted soil displayed an impoverished community, dominated by certain taxa, such as...

Université de Neuchâtel

The Environmental Clade LKM11 and Rozella Form the Deepest Branching Clade of Fungi

Lara, Enrique ; Moreira, David ; López-Garcíaa, Purificación

In: Protist, 2010, vol. 161, no. 1, p. 116-121

Previous environmental surveys of eukaryotic diversity using the small subunit ribosomal RNA (SSU rRNA) gene have revealed many clone sequences that branch near the base of Fungi. In this work, we demonstrate that many of these sequences, including those of the environmental clade LKM11, form a monophyletic and strongly supported group that also includes two sequences derived from the parasitic...

Université de Neuchâtel

Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil

Jousset, Alexandre ; Lara, Enrique ; Nikolausz, Marcell ; Harms, Hauke ; Chatzinotasa, Antonis

In: Science of The Total Environment, 2010, vol. 408, no. 5, p. 1221-1225

Ciliates (or Ciliophora) are ubiquitous organisms which can be widely used as bioindicators in ecosystems exposed to anthropogenic and industrial influences. The evaluation of the environmental impact on soil ciliate communities with methods relying on morphology-based identification may be hampered by the large number of samples usually required for a statistically supported, reliable...

Université de Neuchâtel

Secondary Metabolites Help Biocontrol Strain Pseudomonas fluorescens CHA0 To Escape Protozoan Grazing

Jousset, Alexandre ; Lara, Enrique ; Wall, Luis G. ; Valverde, Claudio

In: Applied and Environmental Microbiology, 2006, vol. 72, no. 11, p. 7083-7090

In soil ecosystems, bacteria must cope with predation activity, which is attributed mainly to protists. The development of antipredation strategies may help bacteria maintain higher populations and persist longer in the soil. We analyzed the interaction between the root-colonizing and biocontrol strain Pseudomonas fluorescens CHA0 and three different protist isolates (an amoeba, a...