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Two- and three-dimensional mid-infrared chemical imaging

Zobi,Fabio ; Obst, Martin

In: CHIMIA International Journal for Chemistry, 2017, vol. 71, no. 1, p. 32–37

This article describes the state-of-the-art in 2D and 3D tomographic mid-IR imaging and its current and potential applications in the imaging of material and biological systems with a focus on cells and tissues. 2D FTIR microscopy is first presented in terms of the basic experimental configurations of the technique, optics, data and image acquisition. This first section provides a basis for...

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Three-dimensional mid-infrared tomographic imaging of endogenous and exogenous molecules in a single intact cell with subcellular resolution

Quaroni, Luca ; Obst, Martin ; Nowak, Marcus ; Zobi, Fabio

In: Angewandte Chemie International Edition, 2014, p. -

Microscopy in the mid-infrared spectral range provides detailed chemical information on a sample at moderate spatial resolution and is being used increasingly in the characterization of biological entities as challenging as single cells. However, a conventional cellular 2D imaging measurement is limited in its ability to associate specific compositional information to subcellular structures...

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