Università della Svizzera italiana

Inhibition of Notch pathway arrests PTEN-deficient advanced prostate cancer by triggering p27-driven cellular senescence

Revandkar, Ajinkya ; Perciato, Maria Luna ; Toso, Alberto ; Alajati, Abdullah ; Chen, Jingjing ; Gerber, Hermeto ; Dimitrov, Mitko ; Rinaldi, Andrea ; Delaleu, Nicolas ; Pasquini, Emiliano ; D’Antuono, Rocco ; Pinton, Sandra ; Losa, Marco ; Gnetti, Letizia ; Arribas, Alberto ; Fraering, Patrick ; Bertoni, Francesco ; Nepveu, Alain ; Andrea Alimonti

In: Nature communications, 2016, vol. 7, p. 13719

Activation of NOTCH signalling is associated with advanced prostate cancer and treatment resistance in prostate cancer patients. However, the mechanism that drives NOTCH activation in prostate cancer remains still elusive. Moreover, preclinical evidence of the therapeutic efficacy of NOTCH inhibitors in prostate cancer is lacking. Here, we provide evidence that PTEN loss in prostate tumours ...

Université de Fribourg

Gpi17p does not stably interact with other subunits of glycosylphosphatidylinositol transamidase in Saccharomyces cerevisiae

Zhu, Yonghua ; Fraering, Patrick ; Vionnet, Christine ; Conzelmann, Andreas

In: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2005, vol. 1735(1), p. 79

Homologues of Gpi8p, Gaa1p, Gpi16p, Gpi17p, and Cdc91p are essential components of the GPI transamidase complex that adds glycosylphosphatidylinositols (GPIs 1) to newly synthesized proteins in the ER. In mammalian cells, these five subunits remain stably associated with each other in detergent. In yeast, we find no stable stoichiometric association of Gpi17p with the Gpi8p–Gpi16p–Gaa1p core...

Université de Fribourg

Functional analysis of the yeast "Saccharomyces cerevisiae" Gpi8 protein and characterization of the purified GPI-transamidase complex

Fraering, Patrick ; Conzelmann, Andreas (Dir.) ; van der Goot, Françoise Gisou (Codir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; no 1354.

Dans le monde des Eucaryotes inférieurs ou supérieurs, de nombreuses glycoprotéines sont ancrées dans leur membrane plasmique par l’intermediaire d’une ancre GPI (glycosylphosphatidylinositol). Cette modification post-traductionnelle est nécessaire pour la croissance et le développement de la levure Saccharomyces cerevisiae. Chez cet organisme, environ 70 cadres ouverts de lecture...