Université de Fribourg

Integrated sensor system for DNA amplification and separation based on thin film technology

Costantini, Francesca ; Petrucci, Giulia ; Lovecchio, Nicola ; Nardecchia, Marco ; Nascetti, Augusto ; Cesare, Giampiero de ; Tedeschi, Lorena ; Domenici, Claudio ; Ruggi, Albert ; Placidi, Pisana ; Scorzoni, Andrea ; Caputo, Domenico

In: IEEE Transactions on Components, Packaging and Manufacturing Technology, 2018, vol. 8, no. 7, p. 1141–1148

This paper presents the development of a lab-on-chip, based on thin-film sensors, suitable for DNA treatments. In particular, the system performs on-chip DNA amplification and separation of double-strand DNA into single-strand DNA, combining a polydimethylsiloxane microfluidic network, thin-film electronic devices, and surface chemistry. Both the analytical procedures rely on the integration...