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Université de Fribourg

Stability of cefiderocol against clinically significant broad-spectrum oxacillinases

Poirel, Laurent ; Kieffer, Nicolas ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2018, vol. 52, no. 6, p. 866–867

Université de Fribourg

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Lescata, Mathilde ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 4, p. 267–269

A rapid (total time <2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a...

Université de Fribourg

Performances of the rapid polymyxin Acinetobacter and Pseudomonas tests for colistin susceptibility testing

Lescat, Mathilde ; Poirel, Laurent ; Jayol, Aurélie ; Nordmann, Patrice

In: Microbial Drug Resistance, 2018, p. -

Owing to the emergence of colistin resistance in nonfermenting Gram negative bacteria, reliable and rapid techniques for testing colistin susceptibility are needed. We evaluated the performances of the Rapid Polymyxin Acinetobacter and Pseudomonas tests using a collection of Acinetobacter baumannii and Pseudomonas aeruginosa clinical isolates.Methods: Colistin susceptibility of A. baumannii...

Université de Fribourg

Screening and characterization of multidrug-resistant gram-negative bacteria from a remote african area, São Tomé and Príncipe

Poirel, Laurent ; Aires-de-Sousa, Marta ; Kudyba, Patrick ; Kieffer, Nicolas ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 9, p. e01021-18

The occurrence of resistance to last-resort antibiotics was evaluated among Enterobacteriaceae isolates recovered from hospitalized children in a remote African archipelago, São Tomé and Príncipe, where there is limited access to those antibiotics. Fifty patients were screened for colonization by carbapenem-, pan- aminoglycoside-, or polymyxin-resistant Enterobacteriaceae. A total of 36...

Université de Fribourg

Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

Jayol, Aurélie ; Kieffer, Nicolas ; Poirel, Laurent ; Guérind, François ; Güneser, Deniz ; Cattoir, Vincent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 90–94

The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin- susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3...

Université de Fribourg

Detection of colistin-resistant Gram-negative rods by using the SuperPolymyxin medium

Jayol, Aurélie ; Poirel, Laurent ; André, Catherine ; Dubois, Véronique ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 95–101

The commercial SuperPolymyxin medium (ELITechGroup, Puteaux, France), currently licensed in Europe, is aimed to screen for either intrinsic or acquired colistin resistance in Gram negatives. It was tested for screening colistin-resistant Gram- negative rods from bacterial cultures of 145 colistin-resistant and 86 colistin- susceptible Gram-negative rods isolates. It exhibited a sensitivity and...

Université de Fribourg

Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil

Kieffer, Nicolas ; Nordmann, Patrice ; Moreno, Andrea Micke ; Zanolli Moreno, Luisa ; Chaby, Richard ; Breton, Aude ; Tissières, Pierre ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 7, p. e00278-18

A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1...

Université de Fribourg

A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria

Nordmann, Patrice ; Mazé, Alain ; Culebras, Esther ; Dobias, Jan ; Jayol, Aurélie ; Poirel, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 91, no. 2, p. 118–122

The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20)....

Université de Fribourg

Carbapenemase-producing organisms: a global scourge

Bonomo, Robert A ; Burd, Eileen M ; Conly, John ; Limbago, Brandi M ; Poirel, Laurent ; Segre, Julie A ; Westblade, Lars F

In: Clinical Infectious Diseases, 2018, vol. 66, no. 8, p. 1290–1297

The dramatic increase in the prevalence and clinical impact of infections caused by bacteria producing carbapenemases is a global health concern. Carbapenemase production is especially problematic when encountered in members of the family Enterobacteriaceae. Due to their ability to readily spread and colonize patients in healthcare environments, preventing the transmission of these organisms...