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Université de Fribourg

Zebrafish caudal fin angiogenesis assay—advanced quantitative assessment including 3-way correlative microscopy

Hlushchuk, Ruslan ; Brönnimann, Daniel ; Shokiche, Carlos Correa ; Schaad, Laura ; Triet, Ramona ; Jazwinska, Anna ; Tschanz, Stefan A. ; Djonov, Valentin

In: PLOS ONE, 2016, vol. 11, no. 3, p. e0149281

Background: Researchers evaluating angiomodulating compounds as a part of scientific projects or pre-clinical studies are often confronted with limitations of applied animal models. The rough and insufficient early-stage compound assessment without reliable quantification of the vascular response counts, at least partially, to the low transition rate to clinics. Objective: To establish an...

Université de Fribourg

Inhibition of Notch signaling induces extensive intussusceptive neo-angiogenesis by recruitment of mononuclear cells

Dimova, Ivanka ; Hlushchuk, Ruslan ; Makanya, Andrew ; Styp-Rekowska, Beata ; Ceausu, Amalia ; Flueckiger, Stefanie ; Lang, Sonja ; Semela, David ; Noble, Ferdinand Le ; Chatterjee, Suvro ; Djonov, Valentin

In: Angiogenesis, 2013, p. 1–17

Notch is an intercellular signaling pathway related mainly to sprouting neo-angiogenesis. The objective of our study was to evaluate the angiogenic mechanisms involved in the vascular augmentation (sprouting/intussusception) after Notch inhibition within perfused vascular beds using the chick area vasculosa and MxCreNotch1(lox/lox) mice. In vivo monitoring combined with morphological...

Université de Fribourg

Decrease in VEGF expression induces intussusceptive vascular pruning

Hlushchuk, Ruslan ; Ehrbar, Martin ; Reichmuth, Philipp ; Heinimann, Niklas ; Styp-Rekowska, Beata ; Escher, Robert ; Baum, Oliver ; Lienemann, Philipp ; Makanya, Andrew ; Keshet, Eli ; Djonov, Valentin

In: Arteriosclerosis, Thrombosis, and Vascular Biology, 2011, vol. 31, p. 2836-2844

Objective—The concept of vascular pruning, the “cuting-off” of vessels, is gaining importance due to expansion of angio-modulating therapies. The proangiogenic effects of vascular endothelial growth factor (VEGF) are broadly described, but the mechanisms of structural alterations by its downregulation are not known.Methods and Results—VEGF165-releasing hydrogels were applied onto the...

Université de Fribourg

Everolimus augments the effects of sorafenib in a syngeneic orthotopic model of hepatocellular carcinoma

Piguet, Anne-Christine ; Saar, Bettina ; Hlushchuk, Ruslan ; St-Pierre, Marie V ; McSheehy, Paul M ; Radojevic, Vesna ; Afthinos, Maresa ; Terracciano, Luigi ; Djonov, Valentin ; Dufour, Jean-Francois J.

In: Molecular Cancer Therapeutics, 2011, p. -

Sorafenib targets the Raf/mitogen activated protein kinase, VEGF and PDGF pathways and prolongs survival patients in advanced hepatocellular carcinoma (HCC). Everolimus inhibits the mammalian target of rapamycin, a kinase overactive in HCC. To investigate whether the antitumor effects of these agents are additive, we compared a combined and sequential treatment regimen of everolimus and sorafenib...

Université de Fribourg

Pulsatile shear and Gja5 modulate arterial identity and remodeling events during flow-driven arteriogenesis

Buschmann, Ivo ; Pries, Axel ; Styp-Rekowska, Beata ; Hillmeister, Philipp ; Loufrani, Laurent ; Henrion, Daniel ; Shi, Yu ; Duelsner, Andre ; Hoefer, Imo ; Gatzke, Nora ; Wang, Haitao ; Lehmann, Kerstin ; Ulm, Lena ; Ritter, Zully ; Hauff, Peter ; Hlushchuk, Ruslan ; Djonov, Valentin ; Veen, Toon van ; Noble, Ferdinand le

In: Development, 2010, vol. 137, p. 2187-2196

In the developing chicken embryo yolk sac vasculature, the expression of arterial identity genes requires arterial hemodynamic conditions. We hypothesize that arterial flow must provide a unique signal that is relevant for supporting arterial identity gene expression and is absent in veins. We analyzed factors related to flow, pressure and oxygenation in the chicken embryo vitelline vasculature...