Université de Fribourg

Multiple cryoinjuries modulate the efficiency of zebrafish heart regeneration

Bise, Thomas ; Sallin, Pauline ; Pfefferli, Catherine ; Jaźwińska, Anna

In: Scientific Reports, 2020, vol. 10, no. 1, p. 11551

Zebrafish can regenerate their damaged hearts throughout their lifespan. It is, however, unknown, whether regeneration remains effective when challenged with successive cycles of cardiac damage in the same animals. Here, we assessed ventricular restoration after two, three and six cryoinjuries interspaced by recovery periods. Using transgenic cell-lineage tracing analysis, we demonstrated...

Université de Fribourg

Towards deciphering variations of heart regeneration in fish

Jaźwińska, Anna ; Blanchoud, Simon

In: Current Opinion in Physiology, 2020, vol. 14, p. 21–26

Among adult vertebrates, the zebrafish presents the rather exceptional capacity to efficiently regenerate its heart after injury. This bony fish has thus become a leading genetic model organism to elucidate the natural mechanisms of successful cardiac restoration. Given its potential biomedical significance, parallel analyses between zebrafish and mammals are aiming at the identification of...

Université de Fribourg

Lymphatic vessels help mend broken hearts

Pfefferli, Catherine ; Jaźwińska, Anna

In: eLife, 2019, vol. 8, p. e52200

Experiments on zebrafish show that the regeneration of the heart after an injury is supported by lymphatic vessels.

Université de Fribourg

Distribution and restoration of serotonin-immunoreactive paraneuronal cells during caudal fin regeneration in zebrafish

König, Désirée ; Dagenais, Paule ; Senk, Anita ; Djonov, Valentin ; Aegerter, Christof M. ; Jaźwińska, Anna

In: Frontiers in Molecular Neuroscience, 2019, vol. 12, p. -

Aquatic vertebrates possess diverse types of sensory cells in their skin to detect stimuli in the water. In the adult zebrafish, a common model organism, the presence of such cells in fins has only rarely been studied. Here, we identified scattered serotonin (5-HT)-positive cells in the epidermis of the caudal fin. These cells were distinct from keratinocytes as revealed by their low...

Université de Fribourg

Zebrafish fin regeneration involves transient serotonin synthesis

König, Désirée ; Jaźwińska, Anna

In: Wound Repair and Regeneration, 2019, p. wrr.12719

The zebrafish is a vertebrate organism capable of regenerating many of its organs. Notably, it can undergo epimorphic regeneration of its fins after amputation. This process occurs through the formation of a wound epithelium and the dedifferentiation of mesenchymal and bone‐forming cells, which form a proliferative blastema. Here, we report that the entry into the regenerative process...

Université de Fribourg

Ciliary neurotrophic factor stimulates cardioprotection and the proliferative activity in the adult zebrafish heart

Bise, Thomas ; de Preux Charles, Anne-Sophie ; Jaźwińska, Anna

In: npj Regenerative Medicine, 2019, vol. 4, no. 1, p. 2

Unlike mammals, adult zebrafish can regenerate their hearts after injury via proliferation of cardiomyocytes. The cell-cycle entry of zebrafish cardiac cells can also be stimulated through preconditioning by thoracotomy, a chest incision without myocardial damage. To identify effector genes of heart preconditioning, we performed transcriptome analysis of ventricles from thoracotomized...

Université de Fribourg

In vivo quantification of mechanical properties of caudal fins in adult zebrafish

Puri, Sahil ; Aegerter-Wilmsen, Tinri ; Jaźwińska, Anna ; Aegerter, Christof M.

In: Journal of Experimental Biology, 2018, vol. 221, no. 4, p. jeb171777

Skip to Next SectionThe caudal fins of adult zebrafish are supported by multiple bony rays that are laterally interconnected by soft interray tissue. Little is known about the fin's mechanical properties that influence bending in response to hydrodynamic forces during swimming. Here, we developed an experimental setup to measure the elastic properties of caudal fins in vivo by applying...

Université de Fribourg

Dynamics of actinotrichia regeneration in the adult zebrafish fin

König, Désirée ; Page, Lionel ; Chassot, Bérénice ; Jaźwińska, Anna

In: Developmental Biology, 2018, vol. 433, no. 2, p. 416–432

The skeleton of adult zebrafish fins comprises lepidotrichia, which are dermal bones of the rays, and actinotrichia, which are non-mineralized spicules at the distal margin of the appendage. Little is known about the regenerative dynamics of the actinotrichia- specific structural proteins called Actinodins. Here, we used immunofluorescence analysis to determine the contribution of two...

Université de Fribourg

The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

Pfefferli, Catherine ; Jaźwińska, Anna

In: Nature Communications, 2017, vol. 8, p. 15151

The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which is induced by TGFβ/Activin-β signalling in the peri-injury zone of the myocardium and the fin mesenchyme. In...

Université de Fribourg

Collagen XII contributes to epicardial and connective tissues in the Zebrafish heart during ontogenesis and regeneration

Marro, Jan ; Pfefferli, Catherine ; Preux Charles, Anne-Sophie de ; Bise, Thomas ; Jaźwińska, Anna

In: PLOS ONE, 2016, vol. 11, no. 10, p. e0165497

Zebrafish heart regeneration depends on cardiac cell proliferation, epicardium activation and transient reparative tissue deposition. The contribution and the regulation of specific collagen types during the regenerative process, however, remain poorly characterized. Here, we identified that the non-fibrillar type XII collagen, which serves as a matrix-bridging component, is expressed in the...