Université de Fribourg

Directing single-molecule emission with dna origami-assembled optical antennas

Hübner, Kristina ; Pilo-Pais, Mauricio ; Selbach, Florian ; Liedl, Tim ; Tinnefeld, Philip ; Stefani, Fernando D. ; Acuna, Guillermo P.

In: Nano Letters, 2019, vol. 19, no. 9, p. 6629–6634

We demonstrate the capability of DNA self-assembled optical antennas to direct the emission of an individual fluorophore, which is free to rotate. DNA origami is used to fabricate optical antennas composed of two colloidal gold nanoparticles separated by a predefined gap and to place a single Cy5 fluorophore near the gap center. Although the fluorophore is able to rotate, its excitation and...

Université de Fribourg

DNA‐mediated self‐assembly of plasmonic antennas with a single quantum dot in the hot spot

Nicoli, Francesca ; Zhang, Tao ; Hübner, Kristina ; Jin, Boyuan ; Selbach, Florian ; Acuna, Guillermo P. ; Argyropoulos, Christos ; Liedl, Tim ; Pilo-Pais, and Mauricio

In: Small, 2019, vol. 15, no. 26, p. 1804418

DNA self‐assembly is a powerful tool to arrange optically active components with high accuracy in a large parallel manner. A facile approach to assemble plasmonic antennas consisting of two metallic nanoparticles (40 nm) with a single colloidal quantum dot positioned at the hot spot is presented here. The design approach is based on DNA complementarity, stoichiometry, and steric hindrance...

Université de Fribourg

Cellular immunostimulation by CpG-sequence-coated DNA origami structures

Schüller, Verena J. ; Heidegger, Simon ; Sandholzer, Nadja ; Nickels, Philipp C. ; Suhartha, Nina A. ; Endres, Stefan ; Bourquin, Carole ; Liedl, Tim

In: ACS Nano, 2011, vol. 5, no. 12, p. 9696–9702

To investigate the potential of DNA origami constructs as programmable and noncytotoxic immunostimulants, we tested the immune responses induced by hollow 30-helix DNA origami tubes covered with up to 62 cytosine-phosphate-guanine (CpG) sequences in freshly isolated spleen cells. Unmethylated CpG sequences that are highly specific for bacterial DNA are recognized by a specialized receptor of the...