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Université de Fribourg

Profiling parvalbumin interneurons using iPSC: challenges and perspectives for Autism Spectrum Disorder (ASD)

Filice, Federica ; Schwaller, Beat ; Michel, Tanja M. ; Grünblatt, Edna

In: Molecular Autism, 2020, vol. 11, no. 1, p. 10

Autism spectrum disorders (ASD) are persistent conditions resulting from disrupted/altered neurodevelopment. ASD multifactorial etiology—and its numerous comorbid conditions—heightens the difficulty in identifying its underlying causes, thus obstructing the development of effective therapies. Increasing evidence from both animal and human studies suggests an altered functioning of the...

Université de Fribourg

Modulation of calretinin expression in human mesothelioma cells reveals the implication of the fak and wnt signaling pathways in conferring chemoresistance towards cisplatin

Wörthmüller, Janine ; Salicio, Valérie ; Oberson, Anne ; Blum, Walter ; Schwaller, Beat

In: International Journal of Molecular Sciences, 2019, vol. 20, no. 21, p. 5391

Malignant mesothelioma (MM) is an aggressive asbestos-linked neoplasm, characterized by dysregulation of signaling pathways. Due to intrinsic or acquired chemoresistance, MM treatment options remain limited. Calretinin is a Ca2+-binding protein expressed during MM tumorigenesis that activates the FAK signaling pathway, promoting invasion and epithelial-to-mesenchymal transition. Constitutive...

Université de Fribourg

Inducible and reversible silencing of the Pvalb gene in mice: An in vitro and in vivo study

Filice, Federica ; Blum, Walter ; Lauber, Emanuel ; Schwaller, Beat

In: European Journal of Neuroscience, 2019, vol. 50, no. 4, p. 2694–2706

Inducible and reversible regulation of gene expression is a powerful approach for unraveling gene functions. Here, we describe the generation of a system to efficiently downregulate in a reversible and inducible manner the Pvalb gene coding for the calcium‐binding protein parvalbumin (PV) in mice. We made use of an IPTG‐inducible short hairpin RNA to activate Pvalb transcript knockdown...

Université de Fribourg

Parvalbumin expression in oligodendrocyte-like CG4 cells causes a reduction in mitochondrial volume, attenuation in reactive oxygen species production and a decrease in cell processes’ length and branching

Lichvarova, Lucia ; Blum, Walter ; Schwaller, Beat ; Szabolcsi, Viktoria

In: Scientific Reports, 2019, vol. 9, no. 1, p. 10603

Forebrain glial cells - ependymal cells and astrocytes -acquire upon injury- a “reactive” phenotype associated with parvalbumin (PV) upregulation. Since free radicals, e.g. reactive oxygen species (ROS) play a role in the pathogenesis of multiple sclerosis, and that PV-upregulation in glial cells is inversely correlated with the level of oxidative stress, we hypothesized that...

Université de Fribourg

Extending the mathematical palette for developmental pattern formation: piebaldism

Dougoud, Michaël ; Mazza, Christian ; Schwaller, Beat ; Pecze, László

In: Bulletin of Mathematical Biology, 2019, vol. 81, no. 5, p. 1461–1478

Here, we present a theoretical investigation with potential insights on developmental mechanisms. Three biological factors, consisting of two diffusing factors and a cell- autonomous immobile transcription factor are combined with different feedback mechanisms. This results in four different situations or fur patterns. Two of them reproduce classical Turing patterns: (1) regularly spaced...

Université de Fribourg

Parvalbumin alters mitochondrial dynamics and affects cell morphology

Lichvarova, Lucia ; Henzi, Thomas ; Safiulina, Dzhamilja ; Kaasik, Allen ; Schwaller, Beat

In: Cellular and Molecular Life Sciences, 2018, vol. 75, no. 24, p. 4643–4666

The Ca2+-binding protein parvalbumin (PV) and mitochondria play important roles in Ca2+ signaling, buffering and sequestration. Antagonistic regulation of PV and mitochondrial volume is observed in in vitro and in vivo model systems. Changes in mitochondrial morphology, mitochondrial volume and dynamics (fusion, fission, mitophagy) resulting from modulation of PV were investigated in MDCK...

Université de Fribourg

Characterization and potential roles of calretinin in rodent spermatozoa

Dressen, Cindy ; Schwaller, Beat ; Vegh, Grégory ; Leleux, Fabienne ; Gall, David ; Lebrun, Philippe ; Lybaert, Pascale

In: Cell Calcium, 2018, vol. 74, p. 94–101

Calretinin has been detected in various excitable cells but the presence and putative roles of such a calcium-binding protein has never been characterized in sperm.Epididymal spermatozoa were collected from C57Bl6 (wild-type, WT) or calretinin knockout (CR−/−) mice and Wistar rats. A specific staining for calretinin was detected by immunofluorescence in the principal piece of the...

Université de Fribourg

Absence of calretinin protein expression in malignant mesotheliomas from asbestos-exposed NF2+/− mice and mouse mesothelioma cell lines from various mouse strains

Blum, Walter ; Henzi, Thomas ; Châtel-Soulet, Hugues-Etienne ; Pecze, Laszlo ; Wörthmüller Rodriguez, Janine ; Vrugt, Bart ; Schwaller, Beat

In: Biomarker Research, 2018, vol. 6, no. 1, p. 19

Calretinin is the most widespread positive marker for the immunohistochemical identification of malignant mesothelioma (MM) and was proposed to serve as a blood- based biomarker. Functionally, evidence has accumulated that calretinin might be implicated in MM tumorigenesis. We aimed to identify calretinin (CR; Calb2) in murine MM and reactive mesothelial cells in granuloma from...

Université de Fribourg

Dysregulation of parvalbumin expression in the Cntnap2−/− mouse model of autism spectrum disorder

Lauber, Emanuel ; Filice, Federica ; Schwaller, Beat

In: Frontiers in Molecular Neuroscience, 2018, vol. 11, p. -

Due to the complex and heterogeneous etiology of autism spectrum disorder (ASD), identification of convergent pathways and/or common molecular endpoints in the pathophysiological processes of ASD development are highly needed in order to facilitate treatment approaches targeted at the core symptoms. We recently reported on decreased expression of the Ca2+-binding protein parvalbumin (PV) in...

Université de Fribourg

Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter

Blum, Walter ; Pecze, László ; Wörthmüller Rodriguez, Janine ; Steinauer, Martine ; Schwaller, Beat

In: BMC Cancer, 2018, vol. 18, p. 475

The calcium-binding protein calretinin (gene name: CALB2) is currently considered as the most sensitive and specific marker for the diagnosis of malignant mesothelioma (MM). MM is a very aggressive tumor strongly linked to asbestos exposure and with no existing cure so far. The mechanisms of calretinin regulation, as well as its distinct function in MM are still poorly understood.